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Abstract
Whole organisms and soluble extracts of a Pseudomonas sp. were examined for their ability to oxidize suspected intermediates in the oxidation of p-isopropyltoluene. A comparison of the relative activities of glucose-grown and p-isopropyltoluene-grown bacteria and their extracts indicated that p-isopropylbenzyl alcohol, p-isopropylbenzaldehyde and p-isopropyl-benzoic acid are intermediates in the oxidation of the hydrocarbon, p-isopropylbenzoic acid was formed during growth on p-isopropyltoluene and this was also shown to be a product of the oxidation of p-isopropyltoluene, p-isopropylbenzyl alcohol and p-isopropylbenzaldehyde by soluble extracts prepared from p-isopropyltoluene-grown bacteria. The enzymes which catalyze the oxidation of p-isopropyltoluene were repressed by glucose and induced by growth on either p-isopropyltoluene or p-isopropylbenzoic acid.
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