1887

Abstract

SUMMARY

Whole organisms and soluble extracts of a sp. were examined for their ability to oxidize suspected intermediates in the oxidation of -isopropyltoluene. A comparison of the relative activities of glucose-grown and -isopropyltoluene-grown bacteria and their extracts indicated that -isopropylbenzyl alcohol, -isopropylbenzaldehyde and -isopropyl-benzoic acid are intermediates in the oxidation of the hydrocarbon, -isopropylbenzoic acid was formed during growth on -isopropyltoluene and this was also shown to be a product of the oxidation of -isopropyltoluene, -isopropylbenzyl alcohol and -isopropylbenzaldehyde by soluble extracts prepared from -isopropyltoluene-grown bacteria. The enzymes which catalyze the oxidation of -isopropyltoluene were repressed by glucose and induced by growth on either -isopropyltoluene or -isopropylbenzoic acid.

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/content/journal/micro/10.1099/00221287-49-3-411
1967-12-01
2021-08-02
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References

  1. Bergmeyer H. U., Bernt E. 1963; Glucose determination with glucose oxidase and peroxidase. Meth. enzymat. Analysis123
    [Google Scholar]
  2. Dagley S., Patel M. D. 1957; Oxidation of p-cresol and related compounds by a Pseudomonas. Biochem. J 66:227
    [Google Scholar]
  3. Davis J. B., Raymond R. L. 1961; Oxidation of alkyl-substituted cyclic hydrocarbons by a Nocardia during growth on n-alkanes. Appl. Microbiol 9:383
    [Google Scholar]
  4. Evans W. C. 1963; The microbiological degradation of aromatic compounds. J. gen. Microbiol 32:177
    [Google Scholar]
  5. Hegeman G. D. 1966; Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. II. Isolation and properties of blocked mutants. J. Bact 91:1155
    [Google Scholar]
  6. Itzhaki R., Gill D. M. 1964; A micro-biuret method for estimating proteins. Analyt. Biochem 9:401
    [Google Scholar]
  7. Yamada K., Horiguchi S., Takahashi J. 1965; Studies on the utilization of hydrocarbons by microorganisms. VI. Screening of aromatic hydrocarbon-assimilating microorganisms and cumic acid formation from p-cymene. Agric. biol. Chem 29:943
    [Google Scholar]
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