1887

Abstract

SUMMARY

Motility was transduced by phage P22 to non-motile ( , etc.) strains, and the flagellar antigens of abortive transductants inferred from the inhibition by antisera of the trails they produced in semi-solid medium. When the recipient had a gene closely linked to (phase-1 flagellar antigen locus) nearly all the abortive transductants evoked from a recipient culture in latent phase 1 manifested the phase-1 antigens of both recipient and donor, whereas those from a culture in latent phase 2 showed neither phase-1 antigen. Thus the expression of an exo-genote allele, like that of the chromosomal allele, was regulated by the phase-determinant of the recipient, at or near (phase-2 flagellar antigen locus). An gene adjacent () to ( activator gene), in the chromosome or in the exogenote, was unexpressed in or abortive transductants in phase 1. This suggests that mutants are -operator-negative mutants.

When the recipient was a phase-1, and therefore non-motile, culture of an or ‘phase-1-curly’ mutant, lysates of phase-2 cultures, but not of phase-1 cultures of the same donor, evoked trails, attributable to abortive transductants. They expressed the donor allele, but not the recipient allele—nor the previously expressed allele of the ‘phase-1-curly’ recipient. It is inferred that a phase-determinant regulates the expression of the gene adjacent () to it (or of which it forms part) but not that of another gene in the same cell; and that it controls the expression of via a represser substance, not via an inducer. The exceptional allele , determines a flagellin of antigenic character ,, apparently identical with that determined by a common allele. , and the common allele were simultaneously expressed in abortive transductants, which suggests that neither flagellin nor messenger RNA functions as the repressor of . It is proposed that an operon, comprising and the structural gene for an repressor substance, has alternative metastable states, ‘on’ in phase 2 and ‘off’ in phase 1, comparable to the wild-type state and to that of an operator-negative mutant.

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1967-11-01
2021-08-04
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