SUMMARY: Studies on the activities of suspensions of and freshly isolated from sheep rumen and washed almost free from bacteria were made by using [C]-amino acids and C-labelled Bacteria were taken up by the protozoa at an approximately linear rate of up to 3000 bacteria/protozoon/hr for 24 hr, after which time at least 40% of the bacterial carbon was no longer in the form of whole bacteria. Arnino acids supplied to the protozoa, either free in the medium or as intact bacteria, were incorporated unchanged into protozoal protein. Addition of individual free C-amino acids to the medium partially inhibited incorporation into protozoal protein of C from labelled with the C form of the same amino acid.


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