1887

Abstract

SUMMARY

The utilization of acetate by blue-green algae was examined and the activities of enzymes involved in its metabolism measured. Although acetate did not stimulate the endogenous respiration of these organisms, the oxidation of acetate was followed by the rate of release of [C] carbon dioxide from [1-C] and [2-C] sodium acetate. Similarly, sodium acetate did not alter the rate of growth of and but in it was found to contribute 7·2% of the dry weight when cultures were gassed with air + CO (95+5, v/v), and 16·9% when gassed with air alone. The presence of acetate in the growth medium did not alter the activity of the acetate-activating enzymes, glyoxylate cycle enzymes or two tricarboxy-lic acid cycle enzymes. The failure to show enzyme adaption by these organisms when supplied with an exogeneous substrate is discussed in relation to their hitherto apparently autotrophic nature.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-49-2-301
1967-11-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/49/2/mic-49-2-301.html?itemId=/content/journal/micro/10.1099/00221287-49-2-301&mimeType=html&fmt=ahah

References

  1. Allen M. B. 1952; The cultivation of Myxophyceae. Arch. Mikrobiol 17:34
    [Google Scholar]
  2. Allison R. K., Skipper H. E., Reid M. R., Short W. A., Hogan G. L. 1953; Studies on the photosynthetic reaction. I. The assimilation of acetate by Nostoc muscorum. J. biol. Chem 204:197
    [Google Scholar]
  3. Butler R. G., Umbreit W. W. 1966; Absorption and utilization of organic matter by the strict autotroph, Thiobacillus thiooxidans, with special reference to aspartic acid. J. Bact 91:661
    [Google Scholar]
  4. Carr N. G. 1966; The occurrence of poly-β-hydroxybutyrate in the blue-green alga, Chloroglea fritschii. Biochim. Biophys. Acta 120:308
    [Google Scholar]
  5. Carr N. G., Hallaway H. M. 1965; Reduction of phenolindo-2,6-dichlorophenol in dark and light by the blue-green alga, Anabaena variabilis. J. gen. Microbiol 39:335
    [Google Scholar]
  6. Carr N. G., Pearce J. 1966; Photoheterotrophism in blue-green algae. Biochem. J 99:28
    [Google Scholar]
  7. Dixon G. H., Kornberg H. L. 1959; Assay methods for key enzymes of the glyoxylate cycle. Biochem. J 72:3
    [Google Scholar]
  8. Fay P. 1965; Heterotrophy and nitrogen fixation in Chlorogloea fritschii. J. gen. Microbiol 39:11
    [Google Scholar]
  9. Friedemann T. E., Haugen G. E. 1943; Pyruvic Acid. II. The determination of keto acids in blood and urine. J. biol. Chem 147:415
    [Google Scholar]
  10. Hoare D. S., Moore R. B. 1965; Photoassimilation of organic compounds by autotrophic blue-green algae. Biochim. Biophys. Acta 109:622
    [Google Scholar]
  11. Ida S., Alexander M. 1965; Permeability of Nitrobader agilis to organic compounds. J. Bact 90:151
    [Google Scholar]
  12. Jones M. E., Lipmann F. 1955; Enzymes of lipid metabolism. Aceto-CoA-kinase. Meth. Enzymol 1:585
    [Google Scholar]
  13. Kiyohara T., Fujita Y., Hattori A., Watanabe A. 1960; Heterotrophic culture of a blue-green alga, Tolypothrix tenuis. I. J. gen. appl. Microbiol 6:176
    [Google Scholar]
  14. Kiyohara T., Fujita Y., Hattori A., Watanabe A. 1962; Effect of light on glucose assimilation in Tolypothrix tenuis. J. gen. appl. Microbiol 8:165
    [Google Scholar]
  15. Kornberg H. L., Elsden S. R. 1961; The metabolism of 2-carbon compounds by micro organisms. Adv. Enzymol 23:401
    [Google Scholar]
  16. Kramer D. N., Klein N., Baselice R. A. 1959; Quantitative determination of glyoxylic acid. Anal. Chem 31:250
    [Google Scholar]
  17. Kratz W. A., Myers J. 1955a; Nutrition and growth of several blue-green algae. Am. J. Bot 42:282
    [Google Scholar]
  18. Kratz W. A., Myers J. 1955b; Photosynthesis and respiration of three blue-green algae. Plant Physiol 30:275
    [Google Scholar]
  19. Layne E. 1957; Spectrophotometric and turbidimetric methods for measuring proteins. III. Biuret Method. Meth. Enzymol 1:450
    [Google Scholar]
  20. Lipmann F., Tuttle L. C. 1945; A specific micromethod for the determination of acyl phosphates. J. biol. Chem 159:21
    [Google Scholar]
  21. Ochoa S. 1948; Biosynthesis of tricarboxylic acids by carbon dioxide fixation. III. Enzymatic mechanisms. J. biol. Chem 174:133
    [Google Scholar]
  22. Ochoa S. 1955; Crystalline condensing enzyme from pig heart. In Meth. Enzymol 1:685
    [Google Scholar]
  23. Pearce J., Carr N. G. 1966; Enzymes of acetate metabolism in blue-green algae. J. gen. Microbiol 45:i
    [Google Scholar]
  24. Smith R. A., Gunsalus I. C. 1957; Isocitratase: Enzyme properties and reaction equilibrium. J. biol. Chem 229:305
    [Google Scholar]
  25. Snyder F., Godfrey P. 1961; Collecting C14O2 in a Warburg flask for subsequent scintillation counting. J. Lipid Res 2:195
    [Google Scholar]
  26. Srere P. A., Brazil H., Gonen L. 1963; The citrate condensing enzyme of pigeon breast muscle and moth flight muscle. Acta Chem. Scand 17:129
    [Google Scholar]
  27. Stadtman E. R. 1952; The purification and properties of phosphotransacetylase. J. biol. Chem 196:527
    [Google Scholar]
  28. Syrett P. J., Merrett M. J., Bocks C. M. 1963; Enzymes of the glyoxylate cycle in Chlorella vulgaris. J. exp. Bot 14:249
    [Google Scholar]
  29. Trüper H. G. 1965; Tricarboxylic acid cycle and related enzymes in Hydrogenomonas strain hi6g+ grown on various carbon sources. Biochim. Biophys. Acta 111:565
    [Google Scholar]
  30. Vernon L. P., Kamen M. D. 1953; Studies on the metabolism of photosynthetic bacteria. XV. Photoautoxidation of ferrocytochrome C in extracts of Rhodospirillum rubrum. Arch. Biochem. Biophys 44:298
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-49-2-301
Loading
/content/journal/micro/10.1099/00221287-49-2-301
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error