1887

Abstract

SUMMARY

Production of -lactamase by nine strains of the genus (eight , one ) was studied to determine its inducibility. Induction was observed with benzylpenicillin (500 g./ml.) in all except one strain. Cultures were examined to locate the enzyme; it was found that in exponential growth the enzyme was cell-bound, and in stationary phase cultures much of it was in the culture medium. Maximum enzyme activity was only demonstrated after cell-breakage. Substrate profiles of crude enzyme preparations were examined and it was observed that the enzymes were 20–80 times more active against cephalosporins than against benzylpenicillin. Evidence is presented which suggests that one strain of produced two -lactamases, an inducible cephalosporinase and a constitutive peni-cillinase.

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/content/journal/micro/10.1099/00221287-49-2-277
1967-11-01
2022-05-16
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References

  1. Abraham E. P., Chain E. B. 1940; An enzyme from bacteria able to destroy penicillin. Nature, Lond 146:837
    [Google Scholar]
  2. Alicino J. F. 1961; Iodometric assay of natural and synthetic penicillins, 6-aminopenicillanic acid and cephalosporin C. Analyt. Chem 33:648
    [Google Scholar]
  3. Ayliffe G. A. J. 1964; Induction of cephalosporinase and penicillinase in Proteus species. Nature, Lond 201:1032
    [Google Scholar]
  4. Ayliffe G. A. J. 1965; Cephalosporinase and penicillinase activity of Gram-negative bacteria. J. gen. Microbiol 40:119
    [Google Scholar]
  5. Citri N., Pollock M. R. 1966; The biochemistry and function of β-lactamase (penicillinase). Advanc. Enzymol 28:237
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria, 1st.61 Cambridge University Press;
    [Google Scholar]
  7. Datta N., Kontomichalou P. 1965; Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature, Lond 208:239
    [Google Scholar]
  8. Datta N., Richmond M. H. 1965; The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli . Biochem. J 98:204
    [Google Scholar]
  9. Fleming P. C., Goldner M., Glass D. G. 1963; Observations on nature, distribution and significance of cephalosporinase. Lancet i:1399
    [Google Scholar]
  10. Hamilton-Miller J. M. T. 1963; Inducible penicillinase in Proteus morgani . Biochem. biophys. Res. Commun 13:43
    [Google Scholar]
  11. Hamilton-Miller J. M. T., Smith J. T., Knox R. 1965; Interaction of cephaloridine with penicillinase-producing Gram-negative bacteria. Nature, Lond 208:235
    [Google Scholar]
  12. Perret C. J. 1954; Iodometric assay of penicillinase. Nature, Lond 174:1012
    [Google Scholar]
  13. Pollock M. R., Torriani A. M. 1953; Purification and physiochemical characteristics of penicillinase from Bacillus cereus. C. r. Séanc. Acacl. Sci., Paris 237:276
    [Google Scholar]
  14. Sabath L. D., Jago M., Abraham E. P. 1965; Cephalosporinase and penicillinase activities of a β-lactamase from Pseudomonas pyocyanea. Biochem. J 96:739
    [Google Scholar]
  15. Smith J. T., Hamilton-Miller J. M. T. 1963; Differences between penicillinases from Gram-positive and Gram-negative bacteria. Nature, Lond 197:976
    [Google Scholar]
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