1887

Abstract

SUMMARY

A mutant growing as long filaments was regularly observed in continuous cultures of strain . The filaments had approximately the same diameter as the parent bacilli and septa were observed at regular intervals, corresponding to the length of single bacilli of the parental type. The links between the individual organisms in the filaments appeared to possess high mechanical strength, as shown by their resistance to ultrasonic treatment. Lysozyme treatment resulted in a complete fragmentation of the filaments into bacilli of the same size as single normal organisms. Electron microscopy showed that the septa of the filaments were thinner than those displayed by dividing normal organisms. A zone of lower electron density, which developed in the contact zones between dividing normal bacilli in the early stages of division, was not observed in the links between the filamentous bacilli.

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/content/journal/micro/10.1099/00221287-49-1-59
1967-10-01
2021-07-30
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References

  1. Barker S. B., Summerson W. H. 1941; The colorimetric determination of lactic acid in biological material. J. biol. Chem 138:535
    [Google Scholar]
  2. Edebo L. 1960; A new press for disruption of micro-organisms and other cells. J. biochem. microbiol. Technol. Engng 2:453
    [Google Scholar]
  3. Friedemann T. E., Haugen G. 1943; Pyruvic acid. II. Determination of keto acids in blood and urine. J. biol. Chem 147:415
    [Google Scholar]
  4. Hughes W. H. 1956; The structure and development of the induced long forms of bacteria. Symp. Soc. gen. Microbiol 6341
    [Google Scholar]
  5. Karnovsky M. J. 1961; Simple methods for ‘staining with lead’ at high pH in electron microscopy. J. biophys. biochem. Cytol 11:729
    [Google Scholar]
  6. Kominek L. A., Halvorson H. O. 1965; Metabolism of poly-β-hydroxy-butyrate and acetoin in Bacillus cereus . J. Bact 90:1251
    [Google Scholar]
  7. Law J. H., Slepecky R. A. 1961; Assay of poly-β-hydroxybutyric acid. J. Bact 82:33
    [Google Scholar]
  8. Levin K., Linde S. 1962; Bestämning av glykos i blod, liquor och urin med ett nytt glykos-oxidasreagens. Svenska Läkartidn 59:3016
    [Google Scholar]
  9. Lundquist P.-G. 1965; The endolymphatic duct and sac in the guinea pig. An electron microscopic and experimental investigation. Acta Oto-lar 201:16
    [Google Scholar]
  10. Murray R. G. E., Steed P., Elson H. E. 1965; The location of the mucopeptide in sections of the cell wall of E. coli and other Gram-negative bacteria. Can. J. Microbiol 11:547
    [Google Scholar]
  11. Ryter A., Kellenberger E. 1958; Étude au microscope electronique de plasmas contenant de l’acide désoxyribonucleique. I. Les nucléotides des baetéries en croissance active. Z. Naturf 13b:597
    [Google Scholar]
  12. Salton M. R. J. 1964 The Bacterial Cell Wall Amsterdam: Elsevier;
    [Google Scholar]
  13. Salton M. R. J., Pavlik J. G. 1960; Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme. Biochim. biophys. Acta 39:398
    [Google Scholar]
  14. Shaforostova L. D. 1962; Dissociation of Bacillus megaterium in flow culture. Microbiology 31:531
    [Google Scholar]
  15. Snell F. D., Snell C. T. 1953 Colorimetric Methods of Analysis III New York: van Nostrand Co;
    [Google Scholar]
  16. Stahl E. 1962 Dünnschichtchromatographie Berlin: Springer Verlag;
    [Google Scholar]
  17. Stolp H., Starr M. P. 1965; Bacteriolysis. A. Rev. Microbiol 19:79
    [Google Scholar]
  18. Watson M. L. 1958; Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol 4:475
    [Google Scholar]
  19. Weidel W., Pelzer H. 1964; Bagshaped macromolecules—a new outlook on bacterial cell walls. Adv. Enzymol 26:193
    [Google Scholar]
  20. Wilkinson J. F. 1958; The extracellular polysaccharides of bacteria. Bact. Rev 22:46
    [Google Scholar]
  21. Williamson D. H., Wilkinson J. F. 1958; The isolation and estimation of the poly-β-hydroxy-butyrate inclusions of Bacillus species. J. gen. Microbiol 19:198
    [Google Scholar]
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