1887

Abstract

SUMMARY: Haemin dependent mutants were isolated from after () ultraviolet irradiation and selection by streptomycin, () exposure of the organisms to copper ions and () treatment with -methyl-'-nitro--nitro-soguanidine. The isolates were grown in a synthetic medium containing hae-min when substances able to neutralize inhibitory factors and to stabilize the medium were included. Two of the mutants were capable of growth in media supplemented only with δ-aminolaevulinic acid, the first intermediate of porphyrin synthesis. Transformation of the mutants to haemin independence was accomplished using deoxyribonucleic acid.

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/content/journal/micro/10.1099/00221287-49-1-31
1967-10-01
2019-10-17
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