1887

Abstract

SUMMARY

‘Whole’ and autolysed motile-phase cells of were examined after negative staining with phosphotungstate. Motile-phase cells and cells in other developmental stages were examined in thin sections. The integument of motile-phase cells was homogeneous and about 30 m thick and was associated with an outer diffuse capsule-like matrix. Three types of intracytoplasmic organelles and occasionally a large fluid-filled vesicle were present. Flagella varied from a few to more than 50 per cell; their diameter was estimated to be approximately 8–9 m. In autolysed cells, the flagella were seen to be attached to differentiated regions of the cytoplasmic membrane. The peripheral zone of the integument of cells in all developmental stages appeared to undergo sloughing. In ‘young hyphal’ and ‘packet’ cells, stratification of the integument was frequently observed. Germinating cells of were larger than motile-phase cells and contained characteristic translucent membraneless cytoplasmic inclusions. The general anatomical features of , especially of germinating cells, closely resembled those of .

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/content/journal/micro/10.1099/00221287-49-1-23
1967-10-01
2021-08-04
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References

  1. Austwick P. K. C. 1958; Cutaneous streptothricosis, mycotic dermatitis and strawberry foot rot and the genus Dermatophilus Van Saceghem. Vet. Revs. Annotat 4:33
    [Google Scholar]
  2. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta 34:103
    [Google Scholar]
  3. Brinton C. C. 1965; The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram-negative bacteria. Trans. N. Y. Acad. Sci 27:1003
    [Google Scholar]
  4. Duguid J. P. 1959; Fimbriae and adhesive properties in Klebsiella strains. J. gen. Microbiol 21:271
    [Google Scholar]
  5. Glauert A. M. 1962; The fine structure of bacteria. Br. med. Bull 18:245
    [Google Scholar]
  6. Glauert A. M., Hopwood D. A. 1961; The fine structure of Streptomyces violaceoruber (S. coelicolor). III. The walls of the mycelium and spores. J. biophys. biochem. Cytol 10:505
    [Google Scholar]
  7. Gordon M. A. 1964; The genus Dermatophilus . J. Bact 88:509
    [Google Scholar]
  8. Gordon M. A., Edwards M. R. 1963; Micromorphology of Dermatophilus congolensis . J. Bact 86:1101
    [Google Scholar]
  9. Karnovsky M. J. 1961; Simple methods for ‘staining with lead’ at high pH in electron microscopy. J. biophys. biochem. Cytol 11:729
    [Google Scholar]
  10. Kawata T., Inoue T. 1965; Ultrastructure of Nocardia asteroides as revealed by electron microscopy. Jap. J. Microbiol 9:101
    [Google Scholar]
  11. Kellenberger E., Ryter A., Séchaud J. 1958; Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. biophys. biochem. Cytol 4:6–1
    [Google Scholar]
  12. Koike M., Takeya K. 1961; Fine structures of intracytoplasmic organelles of mycobacteria. J. biophys. biochem. Cytol 9:597
    [Google Scholar]
  13. Kushida H. 1961; A new embedding method for ultrathin sectioning using a methacrylate resin with three dimensional polymer structure. J. Electron Microsc (Tokyo) 10:194
    [Google Scholar]
  14. Lechevalier H., Holbert P. 1965; Electron microscopic observation of the sporangial structure of a strain of Actinoplanes. J. Bact 89:217
    [Google Scholar]
  15. Millonig G. 1961; A modified procedure for lead staining of thin sections. J. biophys. biochem. Cytol 11:736
    [Google Scholar]
  16. Murray R. G. E. 1963; On the cell wall structure of Spirillum serpens . Can. J. Microbiol 9:381
    [Google Scholar]
  17. Murray R. G. E., Birch-Andersen A. 1963; Specialized structure in the region of the flagella tuft in Spirillum serpens . Can. J. Microbiol 9:393
    [Google Scholar]
  18. Pier A. C., Richard J. L., Farrell E. F. 1964; Fluorescent antibody and cultural techniques in cutaneous streptothricosis. Am. J. vet. Res 25:1014
    [Google Scholar]
  19. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol 17:208
    [Google Scholar]
  20. Rhoades M. E. 1965; Flagellation as a criterion for classification of bacteria. Bact. Rev 29:442
    [Google Scholar]
  21. Roberts D. S. 1961; The life cycle of Dermatophilus dermatonomus the causal agent of ovine mycotic dermatitis. Aust. J. exp. Biol. med. Sci 39:463
    [Google Scholar]
  22. Thompson R. E. M. 1954; A species of Rhizobium isolated from strawberry foot-rot in the sheep. J. Path. Bact 68:445
    [Google Scholar]
  23. Thompson W., D’Arcy. 1942 On Growth and Form, 2. 2470 Cambridge University Press;
    [Google Scholar]
  24. Thornley M. J., Horne R. W. 1962; Electron microscope observations on the structure of fimbriae with particular reference to Klebsiella strains, by the use of the negative staining technique. J. gen. Microbiol 28:51
    [Google Scholar]
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