1887

Abstract

SUMMARY: Extracellular lipase production was a constitutive property of the micrococcus and pseudomonad studied but was considerably influenced by nutritional and physical conditions. The lipase in culture supernatant fluids of the micrococcus was markedly heat resistant but became increasingly more ther-molabile with the degree of purification obtained. The hydrolytic activity of partially purified extracellular lipase preparations from each organism was due to a single protein which was identical with a hydrolytic enzyme also found in cell-free extracts of each organism. The lipases from both organisms had general specificity towards ester linkages although the lipase from the micrococcus was markedly more active towards esters containing short chain fatty acids and comparatively less active towards trigylcerides containing long chain acids than was the pseudomonal lipase. The activity of both lipases showed an optimum for all substrates at pH 8.0-8.5 and did not decrease at higher pH values, indicating the involvement of an acidic group in the enzyme/substrate binding. The results of inhibition studies were consistent with the view that both lipases possess a serine-imidazole active centre and are therefore similar to esterolytic enzymes in mammalian systems.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-48-3-401
1967-09-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/48/3/mic-48-3-401.html?itemId=/content/journal/micro/10.1099/00221287-48-3-401&mimeType=html&fmt=ahah

References

  1. Alford J. A., Pierce D. A. 1963; Production of lipase by Pseudomonas fragi in a synthetic medium. J. Bact. 86:24
    [Google Scholar]
  2. Andrews P. 1965; The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem. J. 96:595
    [Google Scholar]
  3. Augustinsson K.-B. 1961; Multiple forms of esterase in vertebrate blood plasma. Annls. N.Y. Acad. Sci. 94:844
    [Google Scholar]
  4. Barnard E. A., Stein W. O. 1958; The roles of imidazole in biological systems. Adv. Enzymol. 20:51
    [Google Scholar]
  5. Bergmann F., Segal R., Rimon S. 1958; Effect of pH on the activity of eel esterase towards different substrates. Biochem. J. 68:493
    [Google Scholar]
  6. Cohen S., Kurshnick J. B., Purdy C. V. 1953; Observations on mycobacterial esterases with a series of synthetic substrates. J. Bact. 66:266
    [Google Scholar]
  7. Cooper K. E. 1963; The theory of antibiotic inhibition zones. In Analytical Microbiology Ed. by Kavanagh F. Chap I London: Academic Press.;
    [Google Scholar]
  8. Davies R. 1963; Microbial extracellular enzymes, their uses and some factors affecting their formation.. In Biochemistry of Industrial Micro-organisms Ed. by Rainbow C., Rose A. H. Chap. 4 London: Academic Press.;
    [Google Scholar]
  9. Dowd J. E., Riggs D. S. 1965; A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations. J. biol. Chem. 240:863
    [Google Scholar]
  10. Downey W. K., Andrews P. 1965a; Gel filtration applied to the study of lipases and other esterases. Biochem. J. 94:642
    [Google Scholar]
  11. Downey W. K., Andrews P. 1965b; Esterase activity of the non-enzymic proteins of milk and serum. Biochem. J. 96:21c
    [Google Scholar]
  12. Drummond M. C., Tager M. 1959; Enzymatic activity of staphylocoagulase. II. Dissociation of plasma clotting from tributyrinase activity. J. Bact. 78:413
    [Google Scholar]
  13. Fryer T. F., Reiter B., Lawrence R. C. 1967; Lipolytic activity of lactic acid bacteria. J. Dairy Sci. 50:388
    [Google Scholar]
  14. Fryer T. F., Lawrence R. C., Reiter B. 1967; Methods for the isolation and enumeration of lipolytic organisms. Dairy Sci. 50:477
    [Google Scholar]
  15. Gelotte B. 1964; Separation of pancreatic enzymes by gel filtration. Acta chem. scand. 18:1283
    [Google Scholar]
  16. Gutfreund H. 1965 An Introduction to the Study of Enzymes. Oxford: Blackwell Scientific Publications.;
    [Google Scholar]
  17. Hugo W. B., Beveridge E. G. 1962; A quantitative and qualitative study of the lipolytic activity of single strains of seven bacterial species. J. appl. Bact. 25:72
    [Google Scholar]
  18. International Union of Biochemistry 1961. Report of the Commission on Enzymes. Oxford: Pergamon;
    [Google Scholar]
  19. Lampen J. O. 1965; Secretion of enzymes by micro-organisms. Symp. Soc. gen. Microbiol. 15:115
    [Google Scholar]
  20. Lawrence R. C., Fryer T. F., Reiter B. 1967; A rapid method for the quantitative estimation of microbial lipases. Nature, Lond. 213:1264
    [Google Scholar]
  21. Lawrence R. C. 1967; Microbial lipases and related esterases. Dairy Sci. Abstr. 29:1
    [Google Scholar]
  22. Lund B. M. 1965; A comparison by the use of gel electrophoresis of soluble protein components and esterase enzymes of some group D streptococci. J. gen. Microbiol. 40:413
    [Google Scholar]
  23. Mencher J. R., Ng H., Alford J. A. 1965; The extracellular nature of the lipase of Pseudomonas fragi.. Biochim. biophys. Acta 106:628
    [Google Scholar]
  24. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb. 38:732
    [Google Scholar]
  25. Mounter L. A., Tuck K. D. 1956; Dialkylfluorophosphatases of micro-organisms, II. Substrate specificity studies. J. biol. Chem. 221:537
    [Google Scholar]
  26. Nashif S. A., Nelson F. E. 1953; The lipases of Pseudomonas fragi. II. Factors affecting lipase production. J. Dairy Sci. 36:471
    [Google Scholar]
  27. Pollock M. R. 1962; Exoenzymes.. In The Bacteria Ed. by Gunsalus I. C., Stanier R. Y. Vol. 4 p. 121 New York: Academic Press.;
    [Google Scholar]
  28. Rottem S., Razin S. 1964; Lipase activity of mycoplasma. J. gen. Microbiol. 37:123
    [Google Scholar]
  29. Sierra G. 1957; Studies on bacterial esterases. Part I. Differentiation of a lipase and two ali-esterases during the growth of Pseudomonas aeruginosa and some observations on growth and esterase inhibition. Antonie van Leeuwenhoek 23:241
    [Google Scholar]
  30. Smith G. N., Worrell C. S., Swanson A. L. 1949; Inhibition of bacterial esterases by chloramphenicol (chloromycetin). J. Bact. 58:803
    [Google Scholar]
  31. Smith J. L., Alford J. A. 1966; Inhibition of microbial lipases by fatty acids. Appl. Microbiol. 14:699
    [Google Scholar]
  32. Virtanen A. I. 1934; On the enzymes of bacteria and bacterial metabolism. J. Bact. 28:447
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-48-3-401
Loading
/content/journal/micro/10.1099/00221287-48-3-401
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error