SUMMARY: -Methyl-'-nitro--nitrosoguanidine (NTG), which is known to be a very effective mutagen in many systems, induces petite mutations when cells of are treated in acetate buffer. Fifty per cent or more of the survivors may be petite mutants. NTG is a real mutagen in the process since the number of mutants increases at short times of exposure to NTG although the total number of cells decreases. Growing cells are more susceptible to killing and mutation than are cells in buffer. The production of petites may cause difficulties when screening for rare auxotrophs.


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