1887

Abstract

SUMMARY: Serum-sensitive mutants have been derived from serum-resistant smooth virulent and strains by selection for resistance to cephalosporin or penicillin. Chemical analyses of the lipopolysaccharides of these mutants reveal that they belong to at least three different rough or semi-rough classes. Partial or total loss from the lipopolysaccharide of the sugars responsible for O antigenicity resulted in loss of virulence, as well as increased sensitivity to the bactericidal effect of antibody plus complement. However, such loss is not necessary for serum sensitivity because two serum-sensitive mutants possessed lipopolysaccharides indistinguishable from the smooth serum-resistant parents and were nearly as virulent.

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/content/journal/micro/10.1099/00221287-48-2-179
1967-08-01
2021-10-16
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References

  1. Beckmann I., Subbaiah T. V., Stocker B. A. D. 1964; Rough mutants of Salmonella typhimurium. 2. Serological and chemical investigations. Nature, London. 301:1299
    [Google Scholar]
  2. Dische Z. 1953; Qualitative and quantitative colorimetric determination of heptoses. J.biol. Chem. 204:983
    [Google Scholar]
  3. Freeman G. G. 1942; The preparation and properties of a specific polysaccharide from Bact, typhosaTy 2. Biochem. J. 36:340
    [Google Scholar]
  4. Herzberg M., Green J. H. 1964; Characteristics of cell walls of smooth strains of Salmonella typhimurium and derived rough variants. J. gen. Microbiology. 35:421
    [Google Scholar]
  5. Izaki K., Matsuhashi M., Strominger J. L. 1966; Glycopeptidetranspeptidase and D-alanine carboxy-peptidase: a penicillin-sensitive enzymatic reactions. Proc. natn.Acad. Sci., U.S.A. 55:566
    [Google Scholar]
  6. Kabat E. A., Mayer M. M. 1961 Experimental Immunochemistry, 2nd ed.. Springfield, Illinois: C. C. Thomas.;
    [Google Scholar]
  7. Lingelsheim W. v. 1913; ZurFrage der Variation der Typhusbacillen und verwandterGruppen. Zentbl.Bakt.ParasitKde, Abt. I Orig. 68:577
    [Google Scholar]
  8. Lüderitz O., Kauffmann F., Stierlin H., Westphal O. 1960; ZurImmunochemie der O-Antigene von Enterobacteriaceae.II. Vergleich der Zuckerbausteine von Salmonella S-, R-, und T-Formen. Zentbl.Bakt.ParasitKde, Abt. T. Orig. 179:180
    [Google Scholar]
  9. Lüderitz O., Staub A. M., Westphal O. 1966; Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bad.Rev. 30:192
    [Google Scholar]
  10. Lüeritz O., Westphal O. 1966; The significance of enterobacterial mutants for the chemical investigation of their cell-wall polysaccharides. Angew.Chem. int. Edit. 5:198
    [Google Scholar]
  11. Michael J. G., Braun W. 1958; Relationships between bacterial resistance to serum and penicillin. Proc. Soc. exp. Biol. Med. 97:104
    [Google Scholar]
  12. Naede Y., Nikaido H., Makela P. H., Wilkinson R. G., Stocker B. A.D. 1965; Semi-rough strains of Salmonella. Proc. natn.Acad. Sci., U.S.A. 53:147
    [Google Scholar]
  13. Osborn M. J., Rosen S. M., Rothfield L., Zeleznick L. D., Horecker B. L. 1964; Lipopoly-saccharide of the Gram-negative cell wall. Science N.Y.: 145783
    [Google Scholar]
  14. Park J. T., Strominger J. L. 1957; Mode of action of penicillin.Biochemical basis for the mechanism of action of penicillin and for its selective toxicity. Science N. Y.: 12599
    [Google Scholar]
  15. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27:493
    [Google Scholar]
  16. Roantree R. J., Steward J. P. 1965; Mutations to penicillin resistance in Enterobacteriaceae that affect sensitivity to serum and virulence for the mouse. J. Bact. 89:630
    [Google Scholar]
  17. Rogers H. J., Mandelstam J. 1962; Inhibition of cell-wall-mucopeptide formation in Escherichia coli by benzylpenicillin and 6 [d(—)-α-aminophenylacetamido] penicillanic acid (ampicillin). Biochem. J. 84:299
    [Google Scholar]
  18. Rosen S. M., Zeleznick L. D., Fraenkel D., Wiener I. M., Osborn M. J., Horecker B. L. 1965; Characterization of the cell wall Iipopolysaccharide of a mutant of Salmonella typhimurium lacking phosphomannoseisomerase. Biochem. Z. 342:375
    [Google Scholar]
  19. Rowley D. 1956; Rapidly induced changes in the level of nonspecific immunity in laboratory animals. Br. J. exp. Path. 37:223
    [Google Scholar]
  20. Staub A. M. 1960; Bases chimiques de la spécifitéimmunologique des antigenes des Salmonella. Annls Inst. Pasteur, Paris 98:814
    [Google Scholar]
  21. Staub A. M., Tinelli R., Lüderitz O., Westphal O. 1959; Role de quelquessucreset en particular des 3.6dideoxyhexosesdans la specific des antigenes O du tableau de Kauffmann-White. Annls Inst. Pasteur, Paris 96:303
    [Google Scholar]
  22. Steward J. P., Collis L. R., Roantree R. J. 1966; Effects of immunization of guinea pigs lacking bactericidal antibody against Salmonella enteritidis. J. Immunol. 97:224
    [Google Scholar]
  23. Stocker B. A. D., Staub A. M., Tinelli R., Kopacka B. 1960; VI. Eitude de Fantigdne 1 present surdeux Salmonella des groups B et E. Annls Inst. Pasteur, Paris 98:505
    [Google Scholar]
  24. Subbaiah T. V., Stocker B. A. D. 1964; Rough mutants of Salmonella typhimurium. 1. Genetics. Nature, Lond. 201:1298
    [Google Scholar]
  25. ThjØtta R., Waaler E. 1932; Dissociation and sensitiveness to normal serum in dysentery bacilli of type III. J. Bact. 24:301
    [Google Scholar]
  26. Topley W. C. C., Ayrton J. 1924; The segregation of biological factors in B. enteritidis(Aertrycke). J. Hyg., Camb. 22:305
    [Google Scholar]
  27. Westphal O., Luderitz O., Bister F. 1942; über die Extraktion von Bacterienmit Phenol/ Wasser. Z. Naturforsch. 7b:148
    [Google Scholar]
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