RT Journal Article SR Electronic(1) A1 Vise, Ailsa B. A1 Lascelles, JuneYR 1967 T1 Some Properties of a Mutant Strain of Escherichia coli which Requires Lysine and Methionine or Lipoic Acid for Growth JF Microbiology, VO 48 IS 1 SP 87 OP 93 DO https://doi.org/10.1099/00221287-48-1-87 PB Microbiology Society, SN 1465-2080, AB SUMMARY: The requirement of a mutant strain of Escherichia coli for lysine + methionine was due to its inability to make lipoic acid. Aerobic growth of the mutant in minimal medium + lipoic acid was equal to that of the wild-type organism. The factor was replaceable by acetate + succinate. When grown without lipoic acid, suspensions of this organism did not oxidize pyruvate but did so upon addition of the factor; they also accumulated pyruvate from glucose. Extracts from deficient organisms did not oxidize α-ketoglutarate with 3-acetyl-NAD as acceptor. The growth requirements were only exhibited aerobically when provision of acetate + succinate required the operation of the lipoic-dependent pyruvate and α-ketoglutarate oxidase systems, respectively. Anaerobically, these metabolites were formed by lipoic-independent mechanisms, such as fumarate reductase which is repressed by oxygen., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-48-1-87