1887

Abstract

SUMMARY: The requirement of a mutant strain of for lysine + methionine was due to its inability to make lipoic acid. Aerobic growth of the mutant in minimal medium + lipoic acid was equal to that of the wild-type organism. The factor was replaceable by acetate + succinate. When grown without lipoic acid, suspensions of this organism did not oxidize pyruvate but did so upon addition of the factor; they also accumulated pyruvate from glucose. Extracts from deficient organisms did not oxidize α-ketoglutarate with 3-acetyl-NAD as acceptor. The growth requirements were only exhibited aerobically when provision of acetate + succinate required the operation of the lipoic-dependent pyruvate and α-ketoglutarate oxidase systems, respectively. Anaerobically, these metabolites were formed by lipoic-independent mechanisms, such as fumarate reductase which is repressed by oxygen.

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1967-07-01
2024-04-25
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References

  1. Amarasingham C. R., Davis B. D. 1965; Regulation of <x-ketoglutarate dehydrogenase formation in Escherichia coli. J. biol. Chem 240:3664
    [Google Scholar]
  2. Back K. J. C., Westaway E. G. 1962; Studies on a mutant strain of Escherichia coli which requires both methionine and lysine for growth. J. gen. Microbiol 27:41
    [Google Scholar]
  3. Davis B. D., Kornberg H. L., Nagler A., Miller P., Mingioli E. 1959; Formation and functions of succinate in Escherichia coli. Fedn Proc. Fedrt Am. Socs. exp. Biol 18:211
    [Google Scholar]
  4. Ellman G. L. 1959; Tissue sulfhydryl groups. Archs Biochem. Biophys 82:70
    [Google Scholar]
  5. Friedemann T. E., Haugen G. E. 1943; Pjruvic acid. II. The determination of keto acids in blood and urine J. biol. Chem 147:415
    [Google Scholar]
  6. Gounaris A. D., Hager L. P. 1961; A resolution of the Escherichia coli pyruvate dehydrogenase complex. J. biol. Chem 236:1013
    [Google Scholar]
  7. Hager L. P., Gunsalus I. C. 1953; Lipoic acid dehydrogenase: the function of E. coli fraction B. J. Am. chem. Soc 75:5767
    [Google Scholar]
  8. Hager L. P., Kornberg H. L. 1961; On the mechanism of a-oxoglutarate oxidation in Escherichia coli. Biochem. J 78:194
    [Google Scholar]
  9. Henning U. 1963; Ein Regulationsmechanismus beim Abbau der Brenztraubensäure durch Escherichia coli. Biochem. Z 337:490
    [Google Scholar]
  10. Hirsch C. A., Rasminsky M., Davis B. D., Lin E. C. C. 1963; A fumarate reductase in Escherichia coli distinct from succinate dehydrogenase. J. biol. Chem 238:3770
    [Google Scholar]
  11. Huggett A. St. G., Nixon D. A. 1957; Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose. Lancet ii:368
    [Google Scholar]
  12. Lipmann F., Tuttle L. C. 1945; A specific micro method for the determination of acyl phosphates. J. biol. Chem 159:21
    [Google Scholar]
  13. Reed L. J. 1960; Lipoic Acid. In The Enzymes, 2nd ed. Ed. by Boyer P. D., Lardy H., Myrbäck K. 3 p 195 New York: Academic Press Inc;
    [Google Scholar]
  14. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1957; Studies of biosynthesis in Escherichia coli. Pubis Carnegie Instn 10:607
    [Google Scholar]
  15. Rose I. A. 1955; Acetate kinase of bacteria. Meth. Enzymol 1:591
    [Google Scholar]
  16. Rowbury R. J., Woods D. D. 1964; O-succinylhomoserine as an intermediate in the synthesis of cystathionine by Escherichia coli. J. gen. Microbiol 36:341
    [Google Scholar]
  17. Taylor A. L., Thoman M. S. 1964; The genetic map of Escherichia coli k-12. Genetics 50:659
    [Google Scholar]
  18. Wagner A. F., Walton E., Boxer G. E., Pruss M. P., Holly F. W., Folkers K. 1956; Properties and derivatives of a-lipoic acid. J. Am. chem. Soc 78:5079
    [Google Scholar]
  19. Warburg O., Christian W. 1941; Isolierung und Krystallisation des Garungsferments Enolase. Biochem. Z 310:384
    [Google Scholar]
  20. Wyn-Jones R. G., Lascelles J. 1967; The relationship of 4-hydroxybenzoic acid to lysine and methionine formation in Escherichia coli. Biochem. J 103:709
    [Google Scholar]
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