SUMMARY: and were found to incorporate [C]acetate from the growth medium mainly into their polar lipids, whereas and incorporated the acetate mainly into their neutral lipids. var. sp. strain 14 and did not incorporate acetate, although they were found to possess acetokinase activity. Most of the radioactivity of incorporated acetate was found in the fatty acid fraction of lipids. Palmitic and stearic acids almost completely inhibited acetate incorporation by this organism, whereas oleic acid did not, indicating that the major part of the acetate incorporated by was used for the synthesis of saturated fatty acids. Washed required glucose, coenzyme A (CoA) and Mg for acetate uptake. The uptake process was temperature-dependent and pH-dependent and was inhibited by several metabolic inhibitors, in particular iodoacetate. Pyruvate considerably enhanced acetate incorporation into lipids without raising the low degree of radioactivity in the cell fraction soluble in cold trichloroacetic acid. Pyruvate did not replace glucose as an energy source for acetate uptake. Propionate and butyrate markedly decreased the acetate uptake, probably by inhibition of the acetokinase activity of the organisms.


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