1887

Abstract

SUMMARY: and were found to incorporate [C]acetate from the growth medium mainly into their polar lipids, whereas and incorporated the acetate mainly into their neutral lipids. var. sp. strain 14 and did not incorporate acetate, although they were found to possess acetokinase activity. Most of the radioactivity of incorporated acetate was found in the fatty acid fraction of lipids. Palmitic and stearic acids almost completely inhibited acetate incorporation by this organism, whereas oleic acid did not, indicating that the major part of the acetate incorporated by was used for the synthesis of saturated fatty acids. Washed required glucose, coenzyme A (CoA) and Mg for acetate uptake. The uptake process was temperature-dependent and pH-dependent and was inhibited by several metabolic inhibitors, in particular iodoacetate. Pyruvate considerably enhanced acetate incorporation into lipids without raising the low degree of radioactivity in the cell fraction soluble in cold trichloroacetic acid. Pyruvate did not replace glucose as an energy source for acetate uptake. Propionate and butyrate markedly decreased the acetate uptake, probably by inhibition of the acetokinase activity of the organisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-48-1-53
1967-07-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/48/1/mic-48-1-53.html?itemId=/content/journal/micro/10.1099/00221287-48-1-53&mimeType=html&fmt=ahah

References

  1. Ansell G. B., Hawthorne J. N. 1964 Phospholipids p 43 New York: Elsevier Publishing Co;
    [Google Scholar]
  2. Castrejon-Diez J., Fisher T. N., Fisher E. Jun. 1962; Acetokinase reaction in several pleuropneumonia-like organisms. Biochem. biophys. Res. Commun 9:416
    [Google Scholar]
  3. Folch J., Lees M., Sloane-Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem 226:497
    [Google Scholar]
  4. Freundt E. A. 1958; The Mycoplasmataceae. (The Pleuropneumonia Group of Organisms?) Morphology, Biology and Taxonomy Copenhagen: Munksgaard;
    [Google Scholar]
  5. Kates M. 1964; Simplified procedure for hydrolysis or methanolysis of lipids. J. Lipid Res 5:132
    [Google Scholar]
  6. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  7. Neimark H. C., Pickett M. J. 1959; Products of glucose metabolism by pleuropneumonia-like organisms. Ann. N.Y. Acad. Sei 79:531
    [Google Scholar]
  8. Quastel J. H. 1965; Molecular transport at cell membranes. Proc. Roy. Soc B 163:169
    [Google Scholar]
  9. Razin S. 1963; Osmotic lysis of Mycoplasma. J. gen. Microbiol 33:471
    [Google Scholar]
  10. Razin S. 1967; The cell membrane of Mycoplasma. Ann. N.Y. Acad. Sei (in the Press)
    [Google Scholar]
  11. Razin S., Cleverdon R. C. 1965; Carotenoids and cholesterol in membranes of Mycoplasma laidlawii. J. gen. Microbiol 41:409
    [Google Scholar]
  12. Razin S., Cohen A. 1963; Nutritional requirements and metabolism of Mycoplasma laidlawii. J. gen. Microbiol 30:141
    [Google Scholar]
  13. Razin S., Cosenza B. J. 1966; Growth phases of Mycoplasma in liquid media observed with phase-contrast microscope. J. Bact 91:858
    [Google Scholar]
  14. Razin S., Rottem S. 1963; Fatty acid requirements of Mycoplasma laidlawii. J. gen. Microbiol 33:459
    [Google Scholar]
  15. Razin S., Knyszynski A., Lifshitz Y. 1964; Nucleases of Mycoplasma. J. gen. Microbiol 36:323
    [Google Scholar]
  16. Rodwell A. W. 1967; The nutrition and metabolism of Mycoplasma: progress and problems. Ann. N. Y. Acad. Sei (in the Press)
    [Google Scholar]
  17. Rodwell A. W., Abbot A. 1961; The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of Mycoplasma mycoides. J. gen. Microbiol 25:201
    [Google Scholar]
  18. Rose I. A. 1955; Acetate kinase of bacteria (acetokinase). Meth. Enzymol 1:591
    [Google Scholar]
  19. Rottem S., Razin S. 1964; Lipase activity of Mycoplasma. J. gen. Microbiol 37:123
    [Google Scholar]
  20. Rottem S., Razin S. 1966; Adenosine triphosphatase activity of Mycoplasma. J. Bact 92:714
    [Google Scholar]
  21. Smith P. F. 1963; The carotenoid pigments of Mycoplasma. J. gen. Microbiol 32:307
    [Google Scholar]
  22. Smith P. F. 1964; Comparative physiology of pleuropneumonia-like and L type organisms. Bact. Rev 28:97
    [Google Scholar]
  23. Smith P. F., Henrikson C. V. 1965; Comparative biosynthesis of mevalonic acid by Mycoplasma. J. Bact 89:146
    [Google Scholar]
  24. Stadtman E. R. 1955; Phosphotransacetylase from Clostridium kluyveri. Meth. Enzymol 1:596
    [Google Scholar]
  25. Tourtellotte M. E., Morowitz H. J., Kasimer P. 1964; Defined medium for Mycoplasma laidlawii. J. Bact 88:11
    [Google Scholar]
  26. White D., Klein H. P. 1966; Effect of a-glycerophosphate and of palmityl-coenzyme A on lipid synthesis in yeast extract. J. Bact 91:1218
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-48-1-53
Loading
/content/journal/micro/10.1099/00221287-48-1-53
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error