SUMMARY: The rate of utilization of maltose in shaken cultures of a strain of Park Williams 8 was much higher than that of the N source. Considerable gain in toxin yields was achieved when maltose or some intermediates of its metabolism (glucose, glycerophosphate, lactate, acetate) were added to the cultures between 20 and 32 hr of incubation. When, however, the initial concentration of these carbon sources in the medium was increased above the usual value (25 mg./ml.) a decrease in toxin formation resulted. The carbon intermediates were active at concentrations up to 15 times lower than that of maltose. Their action on the toxin titre was immediate, as contrasted to the lag of 3-4 hr which followed the addition of maltose.


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