1887

Abstract

SUMMARY: Morphological, physiological, growth and chemosusceptibility characters were determined for 80 ‘atypical’ acid-fast and 3 non acid-fast strains isolated from tuberculous, or presumed tuberculous, patients, and for 24 control strains belonging to various mycobacterial species. Overall similarity analysis was used for establishing the relationships upon the basis of 60 characters codified by 153 features.

The overall analysis differentiated five clusters having mean matching indices above 80% (80 phenon = 80P), namely: 80P-I (human and bovine mycobacteria); 80P-II (‘atypical’ mycobacteria belonging to the Ist, IInd and IIIrd Runyon groups); 80P-III (mycobacteria related to ); 80P-IV (scotochromogenic mycobacteria with greater metabolic abilities); 80P-V (). Nine strains (among which were three non acid-fast bacilli) were not included in these clusters.

The ‘atypical’ mycobacteria cluster (80P-II) was formed by three clearly delimited subgroups, which broadly corresponded to Runyon's classification.

A more detailed analysis of certain groups, and the determination of the characters of some ‘hypothetical average organisms’, allowed the further differentiation of some subgroups and aberrant strains.

The need to standardize the coding methods is stressed. Because of the value of overall similarity in such a variable genus, an international system to classify and identify strains upon a phenetic basis is suggested.

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1967-07-01
2024-03-28
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References

  1. Beers R. J., Lockhart W. R. 1962; Experimental methods in computer taxonomy. J. gen. Microbiol 28:633
    [Google Scholar]
  2. Beers R. J., Fisher J., Megraw S., Lockhart W. R. 1962; A comparison of methods for computer taxonomy. J. gen. Microbiol 28:641
    [Google Scholar]
  3. Bogdanescu V., Bungetianu G., Racotta R. 1965; Frequency of isolation of atypical Mycobacteria from a restricted area and correlations between the clinico-radiological and bacteriological aspects. 18/A Int. Tuberc. Conf., Munich.
  4. Bojalil L. F., Cerbôn J. 1961; Taxonomic analysis of non-pigmented, rapidly growing Mycobacteria. J. Bact 81:338
    [Google Scholar]
  5. Bojalil L. F., Cerbôn J., Trujillo A. 1962; Adansonian classification of Mycobacteria. J. gen. Microbiol 28:333
    [Google Scholar]
  6. Bonicke R. 1960; Die Klassifizierung ‘atypischer’ Mycobakterien durch Bestimmung ihren unterschiedlichen acylamidatischen Stoffwechselleistungen. Tuberkulosearzt 14:209
    [Google Scholar]
  7. Buraczewska M. 1963; Attempts at classification and identification of atypical bacilli. Gruzlica 31:748
    [Google Scholar]
  8. Canetti G., Rist N., Grosset J. 1963; Mesure de la sensibilité du bacille tuberculeux aux drogues antibacillaires par la méthode des proportions. Rev. Tuberc. Prteumol 27:217
    [Google Scholar]
  9. Cattaneo C., de Rms G. C., Lucchesi M. 1965; Separation of fatty acids from mycobacteria by gas chromatography. 18/A Int. Congr. Tuberc Munich
    [Google Scholar]
  10. Cerbon J., Bojalil L. F. 1961; Physiological relationships of rapidly growing mycobacteria. Adansonian classification. J. gen. Microbiol 25:7
    [Google Scholar]
  11. Cerbon J., Trujillo A. 1963; A comparison of methods for the classification of mycobacteria. Utilization of carbon sources and deamidase tests. Am. Rev. resp. Dis 88:546
    [Google Scholar]
  12. Classification and nomenclature of mycobacteria (1962). Lancet ii:1153
    [Google Scholar]
  13. Collins C. H. 1962; The classification of ‘anonymous’ acid-fast bacilli from human sources. Tubercle, Lortd 43:292
    [Google Scholar]
  14. Colwell R. R., Liston J. 1961; Taxonomic relationships among the pseudomonads. J. Bact 82:1
    [Google Scholar]
  15. Colwell R. R., Mandel M. 1964; Adansonian analysis and deoxyribonucleic acid base composition of some Gram-negative bacteria. J. Bact 87:1412
    [Google Scholar]
  16. Colwell R. R., Mandel M. 1965; Adansonian analysis and deoxyribonucleic acid base composition of Serratia marcescens. J. Bact 89:454
    [Google Scholar]
  17. Floodgate G. D. 1962a; Some comments on the Adansonian taxonomic method. Int. Bull. bact. Nomencl. Taxon 12:171
    [Google Scholar]
  18. Floodgate G. D. 1962b; Some remarks on the theoretical aspects of bacterial taxonomy. Bact. Rev 26:277
    [Google Scholar]
  19. Gilardi E., Hill L. R., Turri M., Silvestri L. G. 1960; Quantitative methods in the systematics of Actinomycetales. I. G. Microbiol 8:203
    [Google Scholar]
  20. Gordon R. E., Smith M. 1953; Rapidly growing, acid-fast bacteria. I. Species description of Mycobacterium phlei Lehmann Neumann and Mycobacterium smegmatis (Trevisan,) Lehmann Neumann J. Bact 66:41
    [Google Scholar]
  21. Hauduroy Hovanessian A., Roussianos D. 1965; Instabilité de la chromogénéité des souches de Mycobacterium kansasii. Ann. Inst. Pasteur 109:142
    [Google Scholar]
  22. Heywood V. H., McNeill J. 1964; Phenetic and phylogenetic classification. Nature, Lond 203:1220
    [Google Scholar]
  23. Hill L. R. 1959; The Adansonian classification of the staphylococci. J. gen. Microbiol 20:277
    [Google Scholar]
  24. Hill L. R., Turri M., Gilardi E., Silvestri L. G. 1961; Quantitative methods in the systematics of Actinomycetales. ü. G. Microbiol 9:56
    [Google Scholar]
  25. Juhlin I. 1960; Methods for the grouping and typing of mycobacteria. Acta path, microbiol. scand 50:177
    [Google Scholar]
  26. Kagramanov A. I. 1963; Sur les mycobactéries acido-résistantes atypiques. Probleme Tuberc 7:69
    [Google Scholar]
  27. Käppler W., Janowiec M. 1963; Untersuchungen über die Identifizireung und Klassifizierung von photochromogenen Mycobakterien (Gruppe I). I. Versuche in vitro Beitr. Klin, Tuberk 126:202
    [Google Scholar]
  28. Kassirskaia N. G., Mencikov D. D. 1965; Facultés immunogéniques des mycobactéries atypiques. Probleme Tuberc no 6:64
    [Google Scholar]
  29. Kendrick W. B., Proctor J. R. 1964; Computer taxonomy in the fungi imperfecti. Can. J. Bot 42:65
    [Google Scholar]
  30. Konno K. 1960; Reliability of the niacin test. Am. Rev. resp. Dis 82:422
    [Google Scholar]
  31. Koyama K. 1964; Experimental studies on immunizing effect of unclassified acid-fast bacilli against tuberculous infection. I. Observations on survival days of mice immunized with various strains of unclassified acid-fast bacilli and infected with bovine type tubercle bacilli Kekkaku 39:64
    [Google Scholar]
  32. Liston J., Wœbe W., Colwell R. R. 1963; Quantitative approach to the study of bacterial species. J. Bact 85:1061
    [Google Scholar]
  33. Lysenko O., Sneath P. H. A. 1959; The use of models in bacterial classification. J. gen. Microbiol 20:284
    [Google Scholar]
  34. Manion R. E., Bradley S. G., Zinneman H. H., Hall W. H. 1964; Interrelationships among Mycobacteria and Nocardiae. J. Bact 87:1056
    [Google Scholar]
  35. Marks J. H., Richards M. 1962; Classification of the anonymous Mycobacteria as a guide to their significance. A preliminary study Mon. Bull. Minist. Hlth 21:200
    [Google Scholar]
  36. Marmur J., Falkow S., Mandel M. 1963; New approaches to bacterial taxonomy. A. Bey. Microbiol 17:329
    [Google Scholar]
  37. Moreluni M., Cattaneo C., Ferri L., Ferrari S., Felicettt L. 1964; Contributto alia risoluzione dei problemi relativi alia tipizzazione dei micobatteri. Nota I, II. Annali 1st. Carlo Forlanini 24:33
    [Google Scholar]
  38. Nasta M., Bogdanescu-Cioclov V. 1959; Le problème des bacilles acido-résistants dits ‘Atypiques’. Bull. Un. int. Tuberc 29:363
    [Google Scholar]
  39. Peizer L., Widelock D. 1955; A colorimetric test for measuring catalase activity of Mycobacterium tuberculosis. Am. Rev. Tuberc 71:807
    [Google Scholar]
  40. Proctor J. R., Kendrick W. B. 1963; Unequal weighting in numerical taxonomy. Nature, Lortd 197:716
    [Google Scholar]
  41. Reynolds K. A. 1965; Numerical taxonomy and comparative elaborateness, with a speculation on unused genes. Nature, Lond 206:166
    [Google Scholar]
  42. Runyon E. H. 1959; The recognition and characterization of pulmonary mycobacterial pathogens other than tubercle bacilli; a review. Bull. Un. int. Tuberc 39:396
    [Google Scholar]
  43. Sattler R. 1963; Numerische Taxonomie. Z. Bot 51:340
    [Google Scholar]
  44. Siebenmann C. O., Barbara C. 1964; Immunologic relationships between typical and atypical mycobacteria as studied by means of the mouse protection test. Am. Rev. resp. Dis 89:20
    [Google Scholar]
  45. Silvestri L. G., Hbll L. R. 1965; Agreement between deoxyribonucleic acid base composition taxometric classification of Gram-positive cocci. J. Bact 90:136
    [Google Scholar]
  46. Silvestri L. G., Turri M., Hill L. R., Gilardi E. 1962; A quantitative approach to the systematics of Actinomycetes based on overall similarity. Symp. Soc. gen. Microbiol 12:333
    [Google Scholar]
  47. Sneath P. H. A. 1957; The application of computers to taxonomy. J. gen. Microbiol 17:201
    [Google Scholar]
  48. Sneath P. H. A. 1962; The construction of taxonomic groups. Symp. Soc. gen. Microbiol 12:289
    [Google Scholar]
  49. Sneath P. H. A., Cowan S. T. 1958; An electro-taxonomic survey of bacteria. J. gen. Microbiol 19:551
    [Google Scholar]
  50. Sokal R. R., Sneath P. H. A. 1963 Principles of Numerical Taxonomy San Francisco: W. H. Freeman and Co;
    [Google Scholar]
  51. Talbot J. M., Sneath P. H. A. 1960; A taxonomic study of Pasteurella septica especially of strains isolated from human sources. J. gen. Microbiol 22:303
    [Google Scholar]
  52. Topley and Wilson's Principles of Bacteriology and Immunity. (1964). , 5th ed. Ed. by Wilson G. S., Miles A. A. London: Arnold;
    [Google Scholar]
  53. Virtanen S. 1960; A study of nitrate reduction by mycobacteria. The use of the nitrate reduction test in the identification of Mycobacteria. Acta tuberc. scand 38:suppl. 48 p 1
    [Google Scholar]
  54. Wayne L. G. 1964; The mycobacterial mystique: deterrent to taxonomy. Am. Rev. resp. Dis 90:255
    [Google Scholar]
  55. Wayne L. G., Doubek J. R. 1963; Differentiation among several types of group III Mycobacteria. Am. Rev. resp. Dis 88:111
    [Google Scholar]
  56. Weissfeiler J., Karassova V., Holland J. 1964; Genetic relationship between ‘ Mycobacterium kansasii’ and ‘Mycobacterium tuberculosis’. Acta microbiol. hung 11:1
    [Google Scholar]
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