1887

Abstract

SUMMARY

Washed suspensions of , grown , incubated anaerobically in the presence of penicillin and neomycin, incorporated single amino acids into the cell protein without conversion to any other amino acid. The C-labelled components of the protozoal ‘pool’ and the medium were also investigated but no extensive catabolism of the amino acids observed. At low external concentrations the amino acids were taken up by an ‘active’ process, but above a critical concentration the amino acids entered the cell by passive diffusion and they have been divided into two groups depending on whether this critical concentration was approximately 0–0001 M or 0–001 M. The rate of amino acid uptake was not altered by the presence of inert particulate matter. Of the 75% of the cell volume occupied by liquid, approximately two-thirds was freely available to amino acids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-3-433
1967-06-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/3/mic-47-3-433.html?itemId=/content/journal/micro/10.1099/00221287-47-3-433&mimeType=html&fmt=ahah

References

  1. Abou Akkada A. R., Howard B. H. 1962; The biochemistry of rumen protozoa. 5. The nitrogen metabolism of Entodinium Biochem. J 82:313
    [Google Scholar]
  2. Borek B. A., Waelsch H. 1953; The enzymatic degradation of histidine. J. biol. Chem 205:459
    [Google Scholar]
  3. Bryant M. P., Robinson I. M. 1963; Apparent incorporation of ammonia and amino acid carbon during growth of selected species of ruminai bacteria. J. Dairy Sci 46:150
    [Google Scholar]
  4. Bull H. B. 1951 Physical Biochemistry New York: John Wiley and Sons Inc;
    [Google Scholar]
  5. Coleman G. S. 1958; Maintenance of oligotrich protozoa from the sheep rumen in vitro. Nature, Lond 182:1104
    [Google Scholar]
  6. Coleman G. S. 1960a; The cultivation of sheep rumen oligotrich protozoa in vitro. J. gen. Microbiol 22:555
    [Google Scholar]
  7. Coleman G. S. 1960b; Effect of penicillin on the maintenance of rumen oligotrich protozoa. Nature, Lond 187:518
    [Google Scholar]
  8. Coleman G. S. 1962; The preparation and survival of almost bacteria-free suspensions of the rumen ciliate Entodinium caudatum. J. gen. Microbiol 28:271
    [Google Scholar]
  9. Coleman G. S. 1963; The growth and metabolism of rumen ciliate protozoa. Symp. Soc. gen. Microbiol 13:198
    [Google Scholar]
  10. Coleman G. S. 1964a; The metabolism of 14C-glycine and 14C-bicarbonate by washed suspensions of the rumen ciliate Entodinium caudatum. J. gen. Microbiol 35:91
    [Google Scholar]
  11. Coleman G. S. 1964b; The metabolism of Escherichia coli and other bacteria by Entodinium caudatum. J. gen. Microbiol 37:209
    [Google Scholar]
  12. Coleman G. S. 1967; The metabolism of amino acids of Escherichia coli and other bacteria by the rumen ciliate Entodinium caudatum. J. gen. Microbiol 47:449
    [Google Scholar]
  13. Coleman G. S., Hall F. J. 1966; Bacterial feeding by the rumen ciliate Entodinium caudatum. J. gen. Microbiol 44:iii
    [Google Scholar]
  14. Conway E. J., Downey M. 1950; An outer metabolic region of the yeast cell. Biochem. J 47:347
    [Google Scholar]
  15. Davson H., Danielli J. F. 1952 The Permeability of Natural Membranes p 54 Cambridge University Press
    [Google Scholar]
  16. Gale E. F. 1947; The assimilation of amino acids by bacteria. 1. The passage of certain amino acids across the cell wall and their concentration in the internal environment of Streptococcus faecalis. J. gen. Microbiol 1:53
    [Google Scholar]
  17. Greenstein J. P., Winitz M. 1961 Chemistry of the Amino Acids New York: John Wiley and Sons Inc;
    [Google Scholar]
  18. Guillaume J., Osteux R. 1955; Détermination des acides aliphatiques volatils de Q à C6 dans les liquids biologiques par chromatographie sur papier. C. r. Séanc. Acad. Sci., Paris 241:501
    [Google Scholar]
  19. Gutierrez J., Davis R. E. 1962; Culture and metabolism of the rumen ciliate Epidinium ecauda-tum (Crawley). Appl. Microbiol 10:305
    [Google Scholar]
  20. Hardy T. L., Holland D. O., Nayler J. H. C. 1955; One-phase solvent mixtures for the separation of amino acids. Analyt. Chem 27:971
    [Google Scholar]
  21. Holter H. 1965; Passage of particles and macromolecules through cell membranes. Symp. Soc. gen. Microbiol 15:89
    [Google Scholar]
  22. Lewis D. 1955; Amino acid metabolism in the rumen of the sheep. Br. J. Nutr 9:215
    [Google Scholar]
  23. Lewis T. R., Emery R. S. 1962; Intermediate products in the catabolism of amino acids by rumen microorganisms. J. Dairy Sci 45:1363
    [Google Scholar]
  24. Menahan L. A., Schultz L. H. 1964; Metabolism of leucine and valine within the rumen. J. Dairy Sci 47:1080
    [Google Scholar]
  25. Mitchell P. 1953; Transport of phosphate across the surface of Micrococcus pyogenes nature of the cell inorganic phosphate. J. gen. Microbiol 9:273
    [Google Scholar]
  26. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies on Biosynthesis in Escherichia coli. Pubis Carnegie Instn no 607
    [Google Scholar]
  27. Tabor H., Mehler A. H. 1954; Isolation of N-formyl L-glutamic acid as an intermediate in the enzymatic degradation of L-histidine. J. biol. Chem 210:559
    [Google Scholar]
  28. Warner A. C. I. 1964; The breakdown of asparagine, glutamine and other amides by microorganisms from the sheep’s rumen. Aust. J. biol. Sci 17:170
    [Google Scholar]
  29. White R. W. 1966; Bacterial population of a rumen ciliate Entodinium caudatum cultured in vitro. J. gen. Microbiol 42:xii
    [Google Scholar]
  30. Williams P. P., Davis R. E., Doetsch R. N., Gutierrez J. 1961; Physiological studies of the rumen protozoon Ophyroscolex caudatus (Eberlein). Appl. Microbiol 9:405
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-3-433
Loading
/content/journal/micro/10.1099/00221287-47-3-433
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error