1887

Abstract

SUMMARY

Tris and EDTA combined to form a homologue which has far greater chelating ability than EDTA alone. The weaker serine and NHCl homo-logues of EDTA replaced EDTA + tris in causing toxicity and permitting lysozyme lysis in . Serine could effectively substitute for tris in these systems for . These compounds had relatively little effect on . The toxicity of EDTA + tris for these organisms could be alleviated by pre-incubation in physiological saline (0–15 M-NaCl). Subsequent lysozyme lysis was prevented by this treatment. The removal of metals from cysts by EDTA + tris was also inhibited. The EDTA + tris + metallo chelate complexes were inactivated (dissociated) by saline.

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1967-06-01
2024-04-20
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References

  1. Brinton C. C., Gemski P., Carnahan J. 1964; A new type of bacterial pilus genetically controlled by the fertility factor of E. coli k 12 and its role in chromosome transfer. Proc. natn. Acad. Sci. U.S.A 52:116
    [Google Scholar]
  2. Burk D., Lineweaver H. 1930; The influence of fixed nitrogen on Azotobacter. J. Bact 19:389
    [Google Scholar]
  3. Eagon R. G., Carson K. J. 1965; Lysis of cell walls and intact cells of Pseudomonas aeruginosa by ethylenediaminetetra-acetic acid and by lysozyme. Can. J. Microbiol 11:193
    [Google Scholar]
  4. Fleming H. P., Ordal Z. J., Steinrauf L. K. 1963; Chelation properties of dipicolinic acid and effects of ionic strength on chelate stability and bacterial spore germination. Bact. Proc p 24
    [Google Scholar]
  5. Goldschmidt M. C., Wyss O. 1966; Chelation effects on Azotobacter cells and cysts. J. Bact 91:120
    [Google Scholar]
  6. Goldschmidt M. C., Goldschmidt E. P., Wyss O. 1967; Differences in toxicity of EDTA among mating types of Escherichia coli. Can. J. Microbiol (in the Press)
    [Google Scholar]
  7. Gray G. W., Wilkinson S. G. 1965a; The action of ethylenediaminetetra-acetic acid on Pseudomonas aeruginosa. J. appl. Bact 28:153
    [Google Scholar]
  8. Gray G. W., Wilkinson S. G. 1965b; The effect of ethylenediaminetetra-acetic acid on the cell walls of some Gram-negative bacteria. J. gen. Microbiol 39:385
    [Google Scholar]
  9. Hayes W. 1964 The Genetics of Bacteria and their Viruses p 567 New York: John Wiley and Sons Inc;
    [Google Scholar]
  10. Herzberg M., Green J. H. 1964; Composition of cell walls of Salmonella. J. gen. Microbiol 35:421
    [Google Scholar]
  11. Jacob F., Wollman E. 1961 Sexuality and the Genetics of Bacteria p 118 New York and London: Academic Press Inc;
    [Google Scholar]
  12. Kaufman B. P., McDonald M. R. 1957; The nature of the changes effected in chromosomal materials by the chelating agent EDTA. Proc. natn. Acad. Sci. U.S.A 43:262
    [Google Scholar]
  13. Leive L. 1965a; A non-specific increase in permeability in Escherichia coli produced by EDTA Proc. natti. Acad. Sei. U.S.A 53:745
    [Google Scholar]
  14. Leive L. 1965b; Actinomycin sensitivity in Escherichia coli produced by EDTA. Biochem. biophys. Res. Comm 18:13
    [Google Scholar]
  15. Maccacaro G. A. 1955; Cell surface and fertility in Escherichia coli. Nature, Lond 176:125
    [Google Scholar]
  16. Maccacaro G. A., Comolli R. 1956; Surface properties correlated with sex compatibility in Escherichia coli. J. gen. Microbiol 15:121
    [Google Scholar]
  17. Martell A. E., Calvin M. 1953a; Chemistry of the Metal Chelate Compounds. p 141 New York: Prentice Hall, Inc;
    [Google Scholar]
  18. Martell A. E., Calvin M. 1953b Chemistry of the Metal Chelate Compounds p 499 New York: Prentice Hall, Inc;
    [Google Scholar]
  19. McQuillen K. 1960a; Bacterial protoplasts. In The Bacteria Ed. by Gunsalus I. C., Stanier R. Y. 6 p 249 New York and London: Academic Press Inc;
    [Google Scholar]
  20. McQuillen K. 1960b; Bacterial protoplasts. In The Bacteria Ed. by Gunsalus I. C., Stanier R. Y. 6 p 345 New York and London: Academic Press Inc;
    [Google Scholar]
  21. Neu H. C., Heppel L. A. 1964; On the surface localization of enzymes in Escherichia coli. Biochem. biophys. Res. Comm 17:215
    [Google Scholar]
  22. Repaske R. 1956; Lysis of gram-negative bacteria by lysozyme. Biochim. biophys. Acta 22:189
    [Google Scholar]
  23. Repaske R. 1958; Lysis of gram-negative organisms and the role of versene. Biochim. biophys. Acta 30:225
    [Google Scholar]
  24. Socolofsky M. D., Wyss O. 1961; Cysts of Azotobacter. J. Bact 81:946
    [Google Scholar]
  25. Voss J. G. 1965; Lysozyme lysis of gram-negative bacteria without production of spheroplasts. J. gen. Microbiol 35:313
    [Google Scholar]
  26. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain b. J. gen. Microbiol 22:158
    [Google Scholar]
  27. Wolin M. J., Rjeichelt E. C. 1964; Lysis of Vibrio succinogenes by EDTA or lysozyme. Bact. Proc p 32
    [Google Scholar]
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