1887

Abstract

SUMMARY

Tris and EDTA combined to form a homologue which has far greater chelating ability than EDTA alone. The weaker serine and NHCl homo-logues of EDTA replaced EDTA + tris in causing toxicity and permitting lysozyme lysis in . Serine could effectively substitute for tris in these systems for . These compounds had relatively little effect on . The toxicity of EDTA + tris for these organisms could be alleviated by pre-incubation in physiological saline (0–15 M-NaCl). Subsequent lysozyme lysis was prevented by this treatment. The removal of metals from cysts by EDTA + tris was also inhibited. The EDTA + tris + metallo chelate complexes were inactivated (dissociated) by saline.

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1967-06-01
2021-07-27
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