1887

Abstract

Strains of isolated from bovine udders were differentiated by comparing biochemical tests, serological typing of proteolytic enzymes, colonial morphology, and the spectrophotometric analysis of pigments extracted with methanol. The cultures were divided into proteinase-positive and proteinase-negative groups, based on their proteolytic activity on skim milk agar, staphylococcus medium no. 110 (Difco), and gelatin. Most of the proteinase-negative cultures produced acetoin, whereas the opposite was true for the proteinase-positive cultures. A further subdivision of the cultures in each group was made by using Baird-Parker's (1963) biochemical subgrouping scheme. The proteinase-positive cultures were also subdivided by serological typing of their proteolytic enzymes into five groups, B, F, G, H, and NR, a non-reacting group. Approximately three-quarters of the cultures in the first four proteinase groups could be placed in one of Baird-Parker's biochemical subgroups. The cultures in the NR group, however, were sufficiently distinctive in their biochemical reactions to be considered as a separate biochemical subgroup. The colonies formed by the cultures were classified into five types, each consisting of two to four forms. No absolute relationship was found between the spectral absorption curves of the pigments and proteinase groups, although there were differences in the types of absorption curves that predominated in each proteinase group. Nine cultures which produced coagulase were more closely related to than to in that they did not utilize mannitol anaerobically or produce α-or β-type haemolysis, they were non-pigmented and were less active biochemically. Also, serological typing of the proteolytic enzymes of three of these cultures resulted in one being classified as group G and two as group H. Group H contained only these two cultures. In contrast 27 cultures of were classified as group A. Except for coagulase production, the biochemical reactions and the serological grouping of the proteolytic enzymes suggested a classification of for the nine cultures.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-2-273
1967-05-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/2/mic-47-2-273.html?itemId=/content/journal/micro/10.1099/00221287-47-2-273&mimeType=html&fmt=ahah

References

  1. Abd-El-Malek Y., Gibson T. 1948; Studies in the bacteriology of milk. II. The staphylococci of milk J. Dairy Res 15:249
    [Google Scholar]
  2. Baird-Parker A. C. 1963; A classification of micrococci and staphylococci based on physiological and biochemical tests. J. gen. Microbiol 30:409
    [Google Scholar]
  3. Baird-Parker A. C. 1965; The classification of staphylococci and micrococci from world-wide sources. J. gen. Microbiol 38:363
    [Google Scholar]
  4. Bergey's Manual of Determinative Bacteriology 1957. , 7th ed. p 445 Baltimore: Williams Wilkins Co;
    [Google Scholar]
  5. Brown R. W. 1966; Color of cultures of Staphylococcus epidermidis determined by spectral reflectance colorimetry. J. Bact 91:911
    [Google Scholar]
  6. Losnegard N., Oeding P. 1963; Immunochemical studies on polysaccharides from Staphylococcus epidermidis . Acta path, microbiol. scand 58:493
    [Google Scholar]
  7. Manual of Microbiological Methods 1957. New York: McGraw-Hill Book Co., Inc;
    [Google Scholar]
  8. Marks J. 1952; Recognition of pathogenic staphylococci: with notes on non-specific staphylococcal haemolysin. J. Path. Bact 64:175
    [Google Scholar]
  9. Page L. A. 1962; Acetylmethylcarbinol production and the classification of aeromonads associated with ulcerative diseases of ectothermic vertebrates. J. Bact 84:772
    [Google Scholar]
  10. Sandvik O. 1962 Studies on Casein Precipitating Enzymes of Aerobic and Facultatively Anaerobic Bacteria p 116 The Veterinary College of Norway, Oslo;
    [Google Scholar]
  11. Sandvik O., Brown R. W. 1965; Spectrophotometric characterization of pigments produced by Staphylococcus epidermidis strains isolated from bovine udders. J. Bact 89:1201
    [Google Scholar]
  12. Sandvik O., Fossum K. 1965; The classification of certain members of the family Micrococcaceae by serologic differentiation of their proteolytic enzymes. Am. J. Vet. Res 26:357
    [Google Scholar]
  13. Shaw C., Stitt J. M., Cowan S. T. 1951; Staphylococci and their classification. J. gen. Microbiol 5:1010
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-2-273
Loading
/content/journal/micro/10.1099/00221287-47-2-273
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error