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Isolated hyphal walls from Phytophthora heveae, Pythium butleri and Saprolegnia ferax were hydrolysed and compared in quantitative composition. A high carbohydrate content was found in all these walls (80–90%); also a small amount of glucosamine (1–2%), lipids (1–2.5%) and protein. Quantitatively the cell walls differed appreciably in protein (1–11%). Five neutral sugars (glucose, galactose, mannose, rhamnose, ribose) were detected by chromatography; glucose was always the main monosaccharide. From the results of acid-extraction of walls and enzymic hydrolysis it was concluded that a portion of cell-wall polysaccharide was cellulose (30–45%). The amino acids found were those typical of normal protein hydrolysates and containing also hydroxyproline.
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