1887

Abstract

Purified suspensions of the sheep rumen organism known colloquially as ‘Quin's oval’ were prepared from sheep rumen liquor. The chemical composition of cell walls prepared from the organisms of these suspensions was found to include protein, lipid and polysaccharide. A fraction from these walls, obtained after removal of much of the protein and polysaccharide, was shown to contain muramic acid, glucosamine and diaminopimelic acid. These findings are consistent with this organism being regarded as a Gram-negative bacterium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-2-207
1967-05-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/2/mic-47-2-207.html?itemId=/content/journal/micro/10.1099/00221287-47-2-207&mimeType=html&fmt=ahah

References

  1. Abou Akkada A. R., Howard B. H. 1960; The biochemistry of rumen protozoa. 3. The carbohydrate metabolism of Entodinium. Biochem. J 76:445
    [Google Scholar]
  2. Allison A. C., Perkins H. R. 1960; Presence of cell walls like those of bacteria in Rickettsiae. Nature, Lond 188:796
    [Google Scholar]
  3. Boas N. F. 1953; Method for the determination of hexosamines in tissues. J. biol. Chem 204:553
    [Google Scholar]
  4. Dische Z., Shettles L. B. 1948; A specific colour reaction of methylpentoses and a spectro-photometric micromethod for their determination. J. biol. Chem 175:595
    [Google Scholar]
  5. Forrester I. T., Wicken A. J. 1966; The chemical composition of the cell walls of some thermophilic bacteria. J. gen. Microbiol 42:147
    [Google Scholar]
  6. Ginger C. D. 1963; Isolation and characterization of muramic acid from two spirochaetes: Borrelia duttoni and Leptospira biflexa . Nature, Lond 199:159
    [Google Scholar]
  7. Grula E. A., Smith G. L., Grula M. M. 1965; Cell division in a species of Erwinia. VIII. Amino acid composition of the mucopeptide in dividing and non-dividing cells Canad. J. Microbiol 11:605
    [Google Scholar]
  8. Ingram M., McGaughey C. A. 1948; Microflora of the rumen of the sheep. Nature, Lond 162:533
    [Google Scholar]
  9. McGaughey C. A., Sellers K. C. 1948; Microflora of the rumen. Nature, Lond 161:1014
    [Google Scholar]
  10. Oxford A. E. 1955; Some observations upon the status of the generic name Selenomonas Prowazek. Int. Bull. bad. Nomencl. Taxon 5:131
    [Google Scholar]
  11. Quin J. I. 1943; Studies on the alimentary tract of Merino sheep in South Africa. VII. Fermentation in the fore stomachs of sheep Onderstepoort J. vet. Sci 18:91
    [Google Scholar]
  12. Salton M. R. J. 1964 The Bacterial Cell Wall London: Elsevier Publishing Co;
    [Google Scholar]
  13. Strominger J. L., Park J. T., Thompson R. E. 1959; Composition of the cell wall of Staphylococcus aureus’ its relation to the mechanism of action of penicillin. J. biol. Chem 234:3263
    [Google Scholar]
  14. Turner N. A., Redgwell R. J. 1966; A mixed layer for the separation of amino acids by thin-layer chromatography. J. Chromat 21:129
    [Google Scholar]
  15. van der Westhuizen G. C. A., Oxford A. E., Quin J. I. 1950; Studies on the alimentary tract of Merino sheep in South Africa. XVI. On the identity of Schizosaccharomyces ovis Part I. Some yeast-like organisms isolated from the rumen contents of sheep fed on a lucerne diet Onderstepoort. J. vet. Sci 24:119
    [Google Scholar]
  16. Wilson C. M. 1959; Quantitative determination of sugars on paper chromatograms. Analyt. Chem 31:1199
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-2-207
Loading
/content/journal/micro/10.1099/00221287-47-2-207
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error