@article{mbs:/content/journal/micro/10.1099/00221287-47-1-87, author = "Brammar, W. J. and Clarke, Patricia H. and Skinner, A. J.", title = "Biochemical and Genetic Studies with Regulator Mutants of the Pseudomonas aeruginosa 8602 Amidase System", journal= "Microbiology", year = "1967", volume = "47", number = "1", pages = "87-102", doi = "https://doi.org/10.1099/00221287-47-1-87", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-47-1-87", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Surmmary Mutants of Pseudomonas aeruginosa strain 8602 were isolated which were unable to produce an aliphatic amidase (acylamide amidohydrolase, EC 3.5.1.4) and could not grow on acetamide as a carbon or nitrogen source. Amidase-constitutive mutants, producing amidase in the absence of inducing amides, were isolated by selection on succinate+formamide agar. Sixteen mutants were magno-constitutive non-inducible mutants producing amidase at about the same rate or greater than the fully induced wild-type strain. Amidase synthesis in one magno-constitutive mutant was repressed by the non-substrate inducer N-acetylacetamide, but the others were not affected in any way. Six mutants were semi-constitutive, producing amidase at 10–50% of the rate of the magno-constitutive mutants and were induced by N-acetylacetamide. Most of the constitutive mutants were very sensitive to catabolite repression by succinate in pyruvate medium, but succinate produced only partial repression of one magno-constitutive mutant and three semi-constitutive mutants; one semi-constitutive mutant was not repressed except in the presence of inducer. Six mutants isolated from succinate + formamide agar had altered inducer specificity and were induced to form amidase by formamide, which is a very poor inducer for the wild-type strain. The formamide-inducible mutants were also sensitive to catabolite repression by succinate although one mutant was only partially repressed. Phage F 116 was used to transduce the amidase structural and regulator genes. In crosses between constitutive mutants of Pseudomonas aeruginosa as donors and amidase-negative mutants as recipients, the two characters were co-transduced with frequencies of 80–100%. Similarly, in crosses between formamide-inducible and amidase-negative mutants these two characters were co-transduced with frequencies of 89–96%. The amidase structural and regulator genes are considered to be closely linked.", }