1887

Abstract

Surmmary

Effects of mutations in three independent proline suppressor loci were studied. Mutants of the locus were almost completely deficient in ornithine transcarbamylase activity and mutants of the locus produced ornithine δ-transaminase constitutively or semiconstitutively; this latter enzyme was strongly induced by arginine in the wild type. The and suppressor mutations were all recessive. Dominant and recessive mutants of the third suppressor locus, , showed higher activities of arginase and ornithine δ-transaminase than did the wild type. It is suggested that in all three cases the enzyme alterations caused by the suppressor mutations allow the synthesis of proline by an alternative route, replacing the blocked major pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-1-77
1967-04-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/1/mic-47-1-77.html?itemId=/content/journal/micro/10.1099/00221287-47-1-77&mimeType=html&fmt=ahah

References

  1. Albrecht A. M., Scher W. I., Vogel H. J. 1962; Determination of aliphatic aldehydes by spectrophotometry. Analyt.Chem. 34:398
    [Google Scholar]
  2. Andersson-Kotto I., Ehrensvard G. 1963; The integrative effect of some genetical and exo-geneous factors upon amino acid formation in Neurosporacrctssa. Hereditas 50:1
    [Google Scholar]
  3. Archibald R. M. 1944; Determination of citrulline and allantoin and demonstration of citrulline in blood plasma. J. biol. Chem. 156:121
    [Google Scholar]
  4. Chinard F. P. 1952; Photometric estimation of proline and ornithine. J. biol. Chem. 199:91
    [Google Scholar]
  5. Cove D. J. 1966; The induction and repression of nitrate reductase in the fungus Aspergillusnidulans. Biochim.biophys. Acta 113:51
    [Google Scholar]
  6. Forbes E. C. 1956; Recombination in the pro region in Aspergillusnidulans. Microb.Genet. Bull. 13:9
    [Google Scholar]
  7. Davis B. D. 1955; Intermediates in amino acid biosynthesis. Adv. Enzymol. 16:247
    [Google Scholar]
  8. Davis R. H. 1962; Consequences of a suppressor gene effective with pyrimidine and proline mutants of Neurospora. Genetics. 47:351
    [Google Scholar]
  9. Irrevekre F. 1965; A modified Sakaguchi spray. Biochim.biophys. Acta 111:551
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
    [Google Scholar]
  11. Middlehoven W. J. 1963; The ornithine pathway in the yeast Candida utilis. Biochim.biophys. Acta 77:152
    [Google Scholar]
  12. Middlehoven W. J. 1964; The pathway of arginine breakdown in Saccharomyces cerevisiae. Biochim.biophys. Acta 93:650
    [Google Scholar]
  13. Pontecorvo G., Roper J. A., Hemmons L. M., MacDonald R. D., Button A. W. J. 1953; The genetics of Aspergillusnidulans. Adv. Genet. 5:141
    [Google Scholar]
  14. Schwartz J. H., Maas W. K. 1960; Analysis of the inhibition of growth produced by cana-vanine in Escherichia colt. J. Bad. 79:794
    [Google Scholar]
  15. Strauss B. S., Pierog S. 1954; Gene interactions: the mode of action of the suppressor of acetate requiring mutants of Neurosporacrassa. J. gen. Microbiol. 10:221
    [Google Scholar]
  16. Strecker H. J. 1957; The interconversion of glutamic acid and proline. I. The formation of pyrro-line carboxylic acid from glutamic acid in Escherichia coli. J. biol. Chem. 225:825
    [Google Scholar]
  17. Vogel H. J. 1955; On the glutamate—proline—ornithine interrelations in various microorganisms.. In Amino Acid Metabolism. Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  18. Vogel R. H., Kopac M. J. 1959; Glutamic y-semialdehyde in arginine and proline synthesis in Neurospora.A mutant-tracer analysis. Biochim.biophys. Acta 36:505
    [Google Scholar]
  19. Vogel R. H., Vogel H. J. 1963; Evidence for acetylated intermediates of arginine synthesis in Neurosporacrassa. Genetics 48:914
    [Google Scholar]
  20. Wagner P. R., Mitchell H. K. 1964 Genetics and Metabolism. New York: John Wiley and Sons Inc.;
    [Google Scholar]
  21. Weglenski P. 1966; Genetical analysis of proline mutants and their suppressors in Aspergillusnidulans. Genet.Res. 8:311
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-1-77
Loading
/content/journal/micro/10.1099/00221287-47-1-77
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error