1887

Abstract

Surmmary

The resistance to nisin was examined for the vegetative forms and the endospores of 14 strains of 9 species of the genus Bacilli, endospores and culture filtrates were tested for ability to inactivate nisin. Marked antinisin activity was detected in extracts of bacilli and endospores of organisms which have a lytic mechanism for rupture of the spore coat (type L); little or no activity was observed in extracts from organisms which rupture the spore coat mechanically (type M). No significant extracellular activity was detected, except after the autolysis of bacilli. When organisms were cultivated in the presence of nisin (50 Reading units/ml.; Berridge, 1949) the yield of organisms and the specific activity of the extracts was decreased; evidence to explain these observations is presented. No quantitative correlation was observed between the production of anti-nisin activity and the resistance of vegetative forms.

The anti-nisin activity of cell-free extracts of bacilli of two species ( and ) was studied further; there was evidence that the activity was probably enzymic. A preliminary study of the properties of the enzymes from these organisms was made. It was found that the anti-nisin enzymes of two strains of differed from the lytic enzymes previously described (Strange & Dark, 1957 ). The anti-nisin enzymes had no effect on polymyxin, gramicidin or bactracin but inactivated subtilin. Proteolytic activity was not observed in the preparations.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-1-33
1967-04-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/1/mic-47-1-33.html?itemId=/content/journal/micro/10.1099/00221287-47-1-33&mimeType=html&fmt=ahah

References

  1. Alifax R., Chevalier R. 1962; Study of the nisinase produced by Streptococcus thermophilus. J. Dairy Res. 29:233
    [Google Scholar]
  2. Berridge N. J. 1949; Preparation of the antibiotic nisin. Biochem. J. 45:486
    [Google Scholar]
  3. Berridge N. J., Newton G. G. F., Abraham E. P. 1952; Purification and nature of the antibiotic nisin. Biochem. J. 52:529
    [Google Scholar]
  4. Bondi A., Dietz C. C. 1948; The susceptibility of penicillinase producing bacteria to penicillin. J. Bact. 55:843
    [Google Scholar]
  5. Carlson S., Bauer H. M. 1957; A study of problems associated with resistance to nisin, an antibiotic produced by Streptococcus lactis. Arch. Hyg. Bakt. 141:445
    [Google Scholar]
  6. Cheeseman G. C., Berridge N. J. 1959; Observations on the molecular weight and chemical composition of nisin A. Biochem. J. 71:185
    [Google Scholar]
  7. Galesloot Th. E. 1956; Lactic acid bacteria which destroy the antibiotic nisin. Ned. Melk-en Zuiveltijdschr. 10:143
    [Google Scholar]
  8. Gould G. W. 1964; Effect of food preservatives on the growth of bacteria from spores. Proc. 4th int. Symp.Food Microbiol. Goteborg, Sweden:17
    [Google Scholar]
  9. Gould G. W., Hitchins A. D. 1965; Germination of spores with Strange & Dark’s lytic enzyme.. In Spores 3:213 ed. by Campbell L. L., Halvorson H. O. Published by Amer. Soc. Microbiol.;
    [Google Scholar]
  10. Gould G. W., Hurst A. 1962; Inhibition of Bacillus spore development by nisin and subtilin. 8th int. Congr.Microbiol.Abstracts A2:11
    [Google Scholar]
  11. Gunsalus I. C. 1954; Extraction of en2ymes from micro-organisms. Meth.Enzymol. 1:51
    [Google Scholar]
  12. Guntelberg A. V., Ottesen M. 1952; Preparation of crystals containing theplakalbumin-forming enzyme from Bacillus subtilis. Nature, Lond. 170:802
    [Google Scholar]
  13. Heinemann B., Williams R. 1966; Inactivation of nisin by pancreatin. J. Dairy Sci. 49:312
    [Google Scholar]
  14. Jarvis B. 1966; Technique for measurement of antibiotic inhibition zones. J. Dairy Sci. 49:318
    [Google Scholar]
  15. Kohn J. 1958; A microelectrophoretic technique. Nature, Lond. 180:39
    [Google Scholar]
  16. Kushner D. J. 1955; The basis of chloramphenicol resistance in Pseudomonas fluorescens. Arch. Biochem. Biophys. 58:347
    [Google Scholar]
  17. Lewis J. C., Snell N. S. 1951; The amino acid composition of subtilin. J. Am. chem. Soc. 73:4812
    [Google Scholar]
  18. Lipinska E., Strzalkowska M. 1959; Experiments on training pure cheese cultures to resist antibiotics produced by Streptococcus lactis.. Proc. 15th int. Dairy Congr. 2:572
    [Google Scholar]
  19. McIlvaine T. C. 1921; A buffer solution for colorimetric comparison. J. biol. Chem. 49:183
    [Google Scholar]
  20. Newton G. G. F., Abraham E. P., Berridge N. J. 1953; Sulphur-containing amino acids of nisin. Nature, Lond. 171:606
    [Google Scholar]
  21. Pollock M. R. 1957; Penicillin-induced resistance to penicillin in cultures of B. cereus.. In Drug Resistance in Micro-organisms. Ed. by Wolstenholme G. E. W., O’Connor E. C. p. 78 London: Churchill;
    [Google Scholar]
  22. Polsinelli M., Ciferri O., Cassani G., Albertini A. 1964; Mechanism of resistance to actinomycin in B. subtilis. J. Bact. 88:1567
    [Google Scholar]
  23. Ramseier H. R. 1960; The action of nisin on Clostridium butyricum. Archiv.Mikrobiol. 37:57
    [Google Scholar]
  24. Report of the Commission on Enzymes of the International Union of Biochemistry 1961 Oxford: Pergamon;
    [Google Scholar]
  25. Sacks S. E. 1955; The tryptic digestion of subtilin. Bact. Proc. A37:30
    [Google Scholar]
  26. Salter D. N. 1959; Purification and properties of a proteinase from B. cereusnctc 945. Biochem. J. 72:23 p.
    [Google Scholar]
  27. Salton M. R. J., Horne R. W. 1951; Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls. Biochim.biophys. Acta 7:177
    [Google Scholar]
  28. Saz A. K., Martinez L. M. 1956; Enzymatic basis of resistance to aureomycin. I. Difference between flavoprotein nitro-reductase of sensitive and resistant Escherichia coli. J. biol. Chem. 223:285
    [Google Scholar]
  29. Smith I. 1960; Chromatographic and Electrophoretic Techniques. , 2nd edn.. Vol. 2: Zone Electrophoresis. London: Heinemann;
    [Google Scholar]
  30. Smith N. R., Gordon R. E., Clark E. E. 1952; Aerobic sporeforming bacteria.U.S.D.A. Agricultural Monograph no. 16
    [Google Scholar]
  31. Strange R. E., Dark F. A. 1957a; A cell-wall lytic enzyme associated with spores of Bacillus species. J. gen. Microbiol. 16:236
    [Google Scholar]
  32. Strange R. E., Dark F. A. 1957b; Cell-wall lytic enzymes at sporulation and germination in Bacillus species. J. gen. Microbiol. 17:525
    [Google Scholar]
  33. Taubman S. B., Young F. E., Corcoran J. W. 1963; Antibiotic glycosides IV.Studies on the mechanism of erythromycin resistance in B. subtilis. Proc. natn.Acad. Sci. U.S.A. 50:955
    [Google Scholar]
  34. Thorpe R. H. 1960; The action of nisin on spoilage bacteria I.The effect of nisin on the heat resistance of Bacillus stearothermophilus. J. appl. Bad. 23:136
    [Google Scholar]
  35. Tramer J., Fowler G. G. 1964; Estimation of nisin in foods. J. Sci. Fd. Agric. 15:522
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-1-33
Loading
/content/journal/micro/10.1099/00221287-47-1-33
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error