1887

Abstract

Surmmary

During germination of spores, the cortex was lost completely, but with spores there was no apparent alteration in the cortex structure. On the other hand, the quantities of dipicolinic acid, calcium and mucopeptide, measured as hexosamine, released from germinating spores were similar to those released from germinating spores. It appears that only about 30% of the mucopeptide of these species is involved in the maintenance of spore dormancy. The solubilization of the spore dipicolinic acid, calcium and mucopeptide during germination only accounted for about half of the loss of dry weight from the spores. During the outgrowth of , the spore coats dissolved away at one pole and the vegetative form grew out, leaving only fragments of the spore integument free in the medium. grew when the largely unaltered coat and cortex layers of the spore split open at the organism's equator.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-1-121
1967-04-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/1/mic-47-1-121.html?itemId=/content/journal/micro/10.1099/00221287-47-1-121&mimeType=html&fmt=ahah

References

  1. Boas N. F. 1953; Method for the determination of hexosamine in tissues. J. biol Chem. 204:953
    [Google Scholar]
  2. Chapman G. B., Zworykin K. A. 1957; Study of germinating Bacillus cereus spores employing television microscopy of living cells and electron microscopy of ultrathin sections. J. Bact. 74:126
    [Google Scholar]
  3. Gould G. W. 1962; Microscopical observations on the emergence of cells of Bacillus spp. from spores under different cultural conditions. J. appl. Bact. 25:35
    [Google Scholar]
  4. Gould G. W., Hitchins A. D. 1965; Germination of spores with Strange and Dark’s spore lytic enzyme.. In Spores. Ed. by Campbell L. L., Halvorson H. O. vol. m: p. 213 Ann Arbor: American Society for Microbiology;
    [Google Scholar]
  5. Immers J., Vasseur E. 1950; Influence of sugars and amines on the colorimetric hexosamine method of Elson and Morgan and its possible elimination. Nature, Lond. 265:898
    [Google Scholar]
  6. Janssen F. W., Lund A. J., Anderson L. E. 1958; Colorimetric assay for dipicolinic acid in bacterial spores. Science N. Y.: 127:26
    [Google Scholar]
  7. Kawata T., Inoue T., Takagi A. 1963; Electron microscopy of spore formation and germination in Bacillus subtilis. Japan. J. Microbiol. 7:23
    [Google Scholar]
  8. Knaysi G. 1965; Further observations on the spodogram of Bacillus cereus endospore. J. Bact. 90:453
    [Google Scholar]
  9. Lamanna C. 1940; The taxonomy of the genus Bacillus. 1. Modes of spore germination. J. Bact. 40:347
    [Google Scholar]
  10. Mayall B. H., Robinow C. F. 1957; Observations with the electron microscope on the organization of the cortex of resting and germinating spores of B. megaterium. J. appl. Bact. 20:333
    [Google Scholar]
  11. Moberley B. J., Shafa F., Gerhardt P. 1966; Structural details of anthrax spores during stages of transformation into vegetative cells. J. Bact. 92:220
    [Google Scholar]
  12. Mollenhauer H. M. 1959; Permanganate fixation of plant cells. J. biophys. biochem. Cytol. 6:431
    [Google Scholar]
  13. Murrell W. G., Warth A. D. 1965; Composition and heat resistance of bacterial spores.. In Spores. Ed. by Campbell L. L., Halvorson H. O. vol. m: p. 1 Ann Arbor: American Society for Microbiology;
    [Google Scholar]
  14. Powell J. F., Strange R. E. 1953; Biochemical changes occurring during the germination of bacterial spores. Biochem. J. 54:205
    [Google Scholar]
  15. Rode L. J., Lewis C. W., Foster J. W. 1962; Electron microscopy of spores of Bacillus megaterium with special reference to the effects of fixation and thin sectioning. J. cell. Biol. 13:423
    [Google Scholar]
  16. Rousseau M., Flechon J., Hermier M. 1966; fitude au microscope 61ectronique de la germination de la spore chez Bacillus subtilis. Ann. Inst. Pasteur 111:149
    [Google Scholar]
  17. Strange R. E., Dark F. A. 1957; A cell-waU lytic enzyme association with spores of Bacillus species. J. gen. Microbiol. 16:236
    [Google Scholar]
  18. Strange R. E., Powell J. F. 1954; Hexosaxnine-containing peptides in spores of Bacillus subtilis B. megaterium and B. cereus. Biochem. J. 58:80
    [Google Scholar]
  19. Thomas R. S. 1964; Ultrastructural localization of mineral matter in bacterial spores by microincineration. J. cell. Biol. 23:113
    [Google Scholar]
  20. Vinter V. 1965; Spores of microorganisms. XVII. The fate of preexisiting diaminopimelic acid-containing structures during germination and postgerminative development of bacterial spores. Folia Microbiol. 10:280
    [Google Scholar]
  21. Warth A. D., Ohye D. F., Murrell W. G. 1963; Location and composition of spore mucopeptide in Bacillus species. J. cell. Biol. 16:593
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-1-121
Loading
/content/journal/micro/10.1099/00221287-47-1-121
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error