1887

Abstract

Surmmary

A strain of , designated M 120, was grown, and produced a marked cytopathic effect (c.p.e.) in calf-, pig-, and monkey-kidney cell cultures. The c.p.e. was characterized by enlargement of the cells, the appearance of intracytoplasmic inclusions and partial destruction of the cell monolayers. A similar c.p.e. was produced in tissue-culture cells following the inoculation of mycoplasma ‘toxins’.

Comparative growth studies of strain M120 in calf-kidney cell cultures and in tissue-culture medium alone showed that the organism grew more readily in the presence of cells. The rate of virus production and appearance of c.p.e. of infectious bovine rhinotracheitis (I.B.R.) virus was delayed in cultures previously infected with the M 120 strain of mycoplasma as compared with normal cultures.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-1-103
1967-04-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/1/mic-47-1-103.html?itemId=/content/journal/micro/10.1099/00221287-47-1-103&mimeType=html&fmt=ahah

References

  1. Afshar A., Stuart P., Huck R. A. 1966; Granular vulvovaginitis (nodular venereal disease) infectious of cattle associated with Mycoplasma bovigenitalium. Vet. Rec. 78:512
    [Google Scholar]
  2. Armstrong J. A., Pereirea H. G., Andrewes C. H. 1961; Observations on the virus of infectious bovine rhinotracheitis, and its affinity with the herpesvirus group. Virology 14:276
    [Google Scholar]
  3. Barile M. F., Malizia W. F., Riggs D. B. 1962; Incidence and detection of pleuropneumonialike organisms in cell cultures by fluorescent antibody and cultural procedures. J. Bact. 84:130
    [Google Scholar]
  4. Butler M. Q., Knight B. C. J.G. 1960; The measurement of the growth of mycoplasma in liquid medium. J. gen. Microbiol. 22:478
    [Google Scholar]
  5. Butler M. Q., Leach R. H. 1964; A mycoplasma which induces acidity and cytopathic effect in tissue culture. J. gen. Microbiol. 34:285
    [Google Scholar]
  6. Carski T. R., Shepard C. C. 1961; Pleuropneumonia-like (mycoplasma) infection of tissue culture. J. Bact. 81:626
    [Google Scholar]
  7. Casterjon-Diez J., Fisher T. N., Fisher E. 1963; Experimental infection of tissue cultures with certain mycoplasma. Proc. Soc. exp. Biol. Med. 112:643
    [Google Scholar]
  8. Clyde W. A. 1963; Studies on Eaton agent in tissue cultures. Am. Rev. resp. Dis. 8:212
    [Google Scholar]
  9. Dawson P. S., Darbyshire J. H., Loosmore,R. M., Paterson A. B., Faull W. B. 1962; Infectious bovine rhinotracheitis(i.b.r.). A clinical condition of cattle occuring in the United Kingdom. Vet. Rec. 74:1379
    [Google Scholar]
  10. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure virus with poliomyelitis virus. J. exp. Med. 99:167
    [Google Scholar]
  11. Edward D. G., ff. & Fitzgerald W. A. 1952; A growth factor needed to isolate organisms of the pleuropneumonia group from the genital tract of cattle. Vet. Rec. 64:395
    [Google Scholar]
  12. Elford W. J. 1931; A new series of graded collodion membranes suitable for general bacteriological use, especially in filterable virus studies. J. Path. Bact. 34:505
    [Google Scholar]
  13. Fabricant C. G., Fabricant J., van Denmark P. J. 1964; Studies on the nutrition and growth requirements of Mycoplasma gallisepticum. J. gen. Microbiol. 35:135
    [Google Scholar]
  14. Gillespie J. H., McEntee K., Kendrick J. W., Wagner W. C. 1959; Comparison of bovine infectious pustularvulvovaginitis virus and infectious bovine rhinotracheitis virus in tissue culture. Can. J. Microbiol. 8:373
    [Google Scholar]
  15. Grumbles L. C., Hall C. F., Cummings G. 1964; Characteristics of avian mycoplasma (p.p.l.o.) in tissue cultures of human and avian cells. Avian Dis. 8:274
    [Google Scholar]
  16. Hayflick L., Stinebring W. R. 1955; Intracellular growth of pleuropneumonia-like organism. Anat. Rec.121
    [Google Scholar]
  17. Hayflick L., Stinebring W. R. 1960; Intracellular growth of pleuropneumonia-like organism (p.p.l.o.) in tissue culture and in ovo. Ann. N. Y. Acad. Sci. 79:433
    [Google Scholar]
  18. Kӓrber G. 1931; BeitragzurKollekrinenBehandlungpharmakologischerReihenversuche. Arch. Exp. Path. Pharmak. 162:480
    [Google Scholar]
  19. Kelton W. H. 1960; Growth studies of pleuropneumonia-like organism. Ann. N. Y. Acad. Sci. 97:422
    [Google Scholar]
  20. Kendrick J. W., Gillespie J. H., McEntee K. 1958; Infectious pustularvulvovaginitis of cattle. Cornell Vet. 48:458
    [Google Scholar]
  21. Kenny G. E., Pollock M. F. 1963; Mammalian cell cultures contaminated with pleuropneumonia-like organisms. I. Effect of PPLO on growth of established cell strains. J. infect. Dis. 112:7
    [Google Scholar]
  22. Kraemer P. M. 1964; Interaction of mycoplasma (PPLO) and murine lymphoma cell cultures. Prevention of cell lysis by arginine. Proc. Soc. exp. Biol. Med. 115:206
    [Google Scholar]
  23. McKercher D. G., Straub O. C., Saito J. K., Wada E. M. 1959; Comparative studies of the etiological agents of infectious bovine rhinotracheitis and infectious pustularvulvovaginitis. Can. J. comp. Med. 23:320
    [Google Scholar]
  24. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb. 38:732
    [Google Scholar]
  25. Nelson J. B. 1960; The behaviour of murine PPLO in HeLa cell cultures. Ann. N. Y. Acad. Sci. 79:450
    [Google Scholar]
  26. Pollock M. E., Kenny G. E., Syverton J. T. 1960; Isolation and elimination of pleuropneumonia-like organisms from mammalian cell cultures. Proc. Soc. exp. Biol. Med. 105:10
    [Google Scholar]
  27. Powelson D. M. 1961; Metabolism of animal cells infected with mycoplasma. J. Bact. 82:288
    [Google Scholar]
  28. Robinson L. B., Wichelhausen R. H., Roizman B. 1956; Contamination of human cell cultures by pleuropneumonia-like organisms. Science 124:1147
    [Google Scholar]
  29. Rothblat G. 1960; PPLO contamination in tissue cultures. Ann. N. Y. Acad. Sci. 79:430
    [Google Scholar]
  30. Rouse H. C., Bonipas V. H., Schlesinger R. W. 1963; Dependence of adenovirus replication on arginine and inhibition of plaque formation by pleuropneumonia-like organism. Virology 20:357
    [Google Scholar]
  31. Rovozzo G. C., Loginbuhl R. E., Helmboldt C. F. 1963; A mycoplasma from a bovine causing cytopathogenic effects in calf kidney tissue culture. Cornell Vet. 53:560
    [Google Scholar]
  32. Schulze I. T., Schlesinger R. W. 1963; Plaque assay of Dengue and other group B arthropod-borne viruses under methylcellulose overlay media. Virology 19:40
    [Google Scholar]
  33. Shepard M. C. 1958; Growth and development of T strains of pleuropneumonia-like organism in human epidermoid carcinoma cells (HeLa). J. Bact. 75:351
    [Google Scholar]
  34. Youngner J. S. 1954; Monolayer tissue culture. I. Preparation and standardization of suspensions of trypsin dispersed monkey kidney cells. Proc. Soc. exp. Biol. Med. 85:202
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-1-103
Loading
/content/journal/micro/10.1099/00221287-47-1-103
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error