1887

Abstract

Surmmary

Cell-free incorporation of amino acid into paramecium protein was accomplished by using ribosomes, soluble fraction, guanosine triphosphate and adenosine triphosphate. Less than 20% of the incorporated label was detected in the soluble fraction, indicating that little if any complete synthesis occurred. Incorporation was markedly decreased by submicrogram concentrations of RNase and by decreasing [Mg] to below 3 μmoles/ ml. A ‘pH 5 fraction’ from mouse liver was able to replace the paramecium soluble fraction but attempts to obtain active ‘pH 5 fractions’ from paramecium failed. Evidence is presented for the presence of polyribosomes in paramecium; there was some indication that they were active in amino acid incorporation. Following incorporation, 80s ribosomes labelled with amino acid were recovered; subjecting these to Mg-deficient buffer caused dissociation into 45s and 30s units. A considerable portion of the label remained with the heavier unit.

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/content/journal/micro/10.1099/00221287-47-1-1
1967-04-01
2019-12-13
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