Skip to content
1887

Microbiology Society logo Microbiology

Volume 46, Issue 3

Fine Structure of Lymphogranuloma Venereum Agent and the Effects of Penicillin and 5-Fluorouracil Free

Abstract

SUMMARY

Growth of lymphogranuloma venereum (LGV) agent in HeLa cells has been studied by light and electron microscopy. Infective elementary bodies enter the host cell by phagocytosis, transform directly into the larger forms known as initial bodies, and multiply by fission within a vesicular cytoplasmic inclusion. Later in the growth cycle, elementary bodies are produced by a process involving internal condensation and a progressive reduction in size of the dividing initial bodies. An envelope with two layers, ribosome-like granules and filamentous ‘nuclear’ material are components common to all developmental forms of the LGV agent. Penicillin prevents multiplication of initial bodies, but not their growth; the very large and vacuolated penicillin forms are similar to bacterial spheroplasts. 5-Fluorouracil affects chiefly the later part of the LGV growth cycle; non-infective forms develop, and the maturation of elementary bodies is blocked. The findings endorse the view that the psittacosis–lymphogranuloma–trachoma group agents are not viruses, but have close affinities with rickettsiae and certain bacteria.

  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-46-3-435
1967-03-01
2025-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/46/3/mic-46-3-435.html?itemId=/content/journal/micro/10.1099/00221287-46-3-435&mimeType=html&fmt=ahah

References

  1. Anderson, D. R.,, Hopps, H. E.,, Barile, M. F., & Bernheim, B. C..( 1965;). Comparison of the ultra-structure of several Rickettsiae, Ornithosis virus and Mycoplasma in tissue culture. . J. Bact. 90, 1387.
     [Google Scholar]
  2. Andrewes, C. H..( 1964). Viruses of Vertebrates. London:: Baillière, Tindall and Cox;.
     [Google Scholar]
  3. Armstrong, J. A., & Reed, S. E..( 1964;). Nature and origin of initial bodies in lymphogranuloma venereum. . Nature, Lond. 201, 371.
     [Google Scholar]
  4. Armstrong, J. A.,, Valentine, R. C., & Fildes, C.. ( 1963;). Structure and replication of the trachoma agent in cell cultures as shown by electron microscopy. . J. gen. Microbiol. 30, 59.
     [Google Scholar]
  5. Bedson, S. P..( 1959;). The psittacosis-lymphogranuloma group of infective agents. . Jl R. lust. publ. Hlth 22, 131.
     [Google Scholar]
  6. Bedson, S. P., & Gostling, J. V. T.. ( 1954;). A study of the mode of multiplication of psittacosis virus. . Br. J. exp. Path. 35, 299.
     [Google Scholar]
  7. Bedson, S. P.,, Western, G. T., & Levy-Simpson, S.. ( 1930;). Observations on the aetiology of psittacosis. . Lancet i, 235, 345.
     [Google Scholar]
  8. Bernkopf, H.,, Mashiah, P., & Becker, Y.. ( 1962;). Correlation between morphological and biochemical changes and the appearance of infectivity in FL cell cultures infected with trachoma agent. . Ann, N.Y. Acad. Sci. 98, Art. 1 , 62.
     [Google Scholar]
  9. Bladen, H. A., & Waters, J. F..( 1963;). Electron microscopic study of some strains of Bacteriodes. . J. Bact. 86, 1339.
     [Google Scholar]
  10. Cohen, S. S.,, Flaks, J. G.,, Barnes, H. D.,, Loeb, M. R., & Lichtenstein, J.. ( 1958;). The mode of action of 5-fluorouracil and its derivatives. . Proc. natn. Acad. Sci., U.S.A. 44, 1004.
     [Google Scholar]
  11. Conti, S. F., & Gettner, M. E..( 1962;). Electron microscopy of cellular division in Escherichia coli. . J. Bact. 83, 544.
     [Google Scholar]
  12. Erlandson, R. A., & Allen, E. G..( 1964;). The ultrastructure of meningopneumonitis. . Virology 22, 410.
     [Google Scholar]
  13. Findlay, G. M.,, Mackenzie, R. D., & MacCallum, F. O..( 1938;). A morphological study of the virus of lymphogranuloma inguinale (climatic bubo). . Trans. R. Soc. trop. Med. Hyg. 32, 183.
     [Google Scholar]
  14. Furness, G.,, Graham, D. M., & Reeve, P.. ( 1960;). The titration of trachoma and inclusion blenorrhoea viruses in cell cultures. . J. gen. Microbiol. 23, 613.
     [Google Scholar]
  15. Gaylord, W. H..( 1954;). Intracellular forms of meningopneumonitis virus. . J. exp. Med. 100, 575.
     [Google Scholar]
  16. Higashi, N.. ( 1959;). Electron microscopy of viruses in thin sections of cells grown in culture. . Progr. med. Virol. 2, 43.
     [Google Scholar]
  17. Higashi, N.. ( 1964;). Mode of reproduction of the psittacosis-lymphogranulomatrachoma (PLT) group viruses. . Internat. Rev. exp. Path. 3, 35.
     [Google Scholar]
  18. Higashi, N.. ( 1965;). Electron microscopic studies on the mode of reproduction of trachoma virus and psittacosis virus in cell cultures. . Exp. molec. Path. 4, 24.
     [Google Scholar]
  19. Higashi, N.,, Tamura, A., & Iwanaga, M.. ( 1962;). Developmental cycle and reproductive mechanism of the meningopneumonitis virus in strain L cells. . Ann. N.Y. Acad. Sci. 98, Art. 1 , 100.
     [Google Scholar]
  20. Hines, W. D.,, Freeman, B. A., & Pearson, G. R..( 1964;). Fine structure of Brucella suis spheroplasts. . J. Bact. 87, 1492.
     [Google Scholar]
  21. Horowitz, J.,, Saukkonen, J. J., & Chargaff, E.. ( 1960;). Effects of fluoropyrimidines on the synthesis of bacterial proteins and nucleic acids. . J. biol. Chem. 235, 3266.
     [Google Scholar]
  22. Ito, S., & Vinson, J. W..( 1965;). Fine structure of Rickettsia quintana cultivated in vitro and in the louse. . J. Bact. 89, 481.
     [Google Scholar]
  23. Kajima, M.,, Sharon, N., & Pollard, M.. ( 1964;). Electron microscopy of latent psittacosis virus in McCoy cells. . J. Bact. 88, 709.
     [Google Scholar]
  24. Kellenberger, E.,, Ryter, A., & Séchaud, J.. ( 1958;). Electron microscopic studies of DNA-con-taining plasma. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. . J. biophys. biochem. Cytol. 4, 671.
     [Google Scholar]
  25. Lederberg, J., & St Clair, J.. ( 1958;). Protoplasts and L-type growth of Escherichia coli. . J. Bact. 75, 143.
     [Google Scholar]
  26. Litwin, J.,, Officer, J. E.,, Brown, A., & Moulder, J. W..( 1961;). A comparative study of the growth cycles of different members of the psittacosis group in different host cells. . J. infect. Dis. 109, 251.
     [Google Scholar]
  27. Martin, H. H..( 1963;). Bacterial protoplasts—a review. . J. theoret. Biol. 5, 1.
     [Google Scholar]
  28. McQuillen, K.. ( 1960). In The Bacteria, vol. i. Ed. by Gunsalus I. C, and Stanier R. Y... New York and London:: Academic Press;.
     [Google Scholar]
  29. Mitsui, Y.,, Fujimoto, M., & Kajima, M.. ( 1964;). Development and morphology of trachoma agent in the yolk sac as revealed by electron microscopy. . Virology 23, 30.
     [Google Scholar]
  30. Mitsui, Y.,, Suzuki, A.,, Hanabusa, J.,, Minoda, R.,, Ogata, S.,, Fukushima, S., & Miura, M.. ( 1958;). Structures of the initial bodies of trachoma inclusion as revealed in section by electron microscopy. . Virology 6, 137.
     [Google Scholar]
  31. Moulder, J. W..( 1964). The Psittacosis Group as Bacteria. New York:: John Wiley and Sons, Inc;.
     [Google Scholar]
  32. Palade, G. E..( 1952;). A study of fixation for electron microscopy. . J. exp. Med. 95, 285.
     [Google Scholar]
  33. Perkins, H. R., & Allison, A. C..( 1963;). Cell wall constituents of rickettsiae and psittacosis-lympho- granuloma organisms. . J. gen. Microbiol. 30, 469.
     [Google Scholar]
  34. Pollard, M., & Starr, T.. ( 1962;). Study of intracellular virus with acridine orange fluorochrome. . Progr. med. Virol. 4, 54.
     [Google Scholar]
  35. Pollard, M.,, Starr, T.,, Tanami, Y., & Elliott, A.. ( 1960;). Cytochemical effects of substituted pyrimidines on psittacosis virus. . Proc. Soc. exp. Biol. Med. 105, 476.
     [Google Scholar]
  36. Reynolds, E. S..( 1963;). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. . J. Cell Biol. 17, 208.
     [Google Scholar]
  37. Rogers, H. J., & Perkins, H. R..( 1960;). 5-Fluorouracil and mucopeptide biosynthesis by Staphy-lococcus aureus. . Biochem. J. 77, 448.
     [Google Scholar]
  38. Tajima, M.,, Nomura, Y., & Kubota, Y.. ( 1957;). Structure and development of viruses of the psitta- cosis-lymphogranuloma group observed in the electron microscope. . J. Bact. 74, 605.
     [Google Scholar]
  39. Tanami, Y.,, Pollard, M., & Starr, J. J..( 1961;). Replication pattern of psittacosis virus in a tissue culture system. . Virology 15, 22.
     [Google Scholar]
  40. Thorsson, K. G., & Weibull, C.. ( 1958;). Studies on the structure of bacterial L-forms, protoplasts and protoplast-like bodies. . J. Ultrastr. Res. 1, 412.
     [Google Scholar]
  41. Tomasz, A., & Borek, E.. ( 1960;). The mechanism of bacterial fragility produced by 5-fiuorouracil; the accumulation of cell wall precursors. . Proc. natn. Acad. Sci., U.S.A. 46, 324.
     [Google Scholar]
  42. Weibull, C., & Mohri, T.. ( 1965;). Fixation of bacterial L-forms for electron microscopy. . J. Bact. 89, 901.
     [Google Scholar]
  43. Weiss, E.. ( 1950;). The effect of antibiotics on agents of the psittacosis-LGV group. I. The effect of penicillin. . J. infect. Dis. 87, 249.
     [Google Scholar]
  44. Weiss, E.. ( 1955;). The nature of the psittacosis-lymphogranuloma group of micro-organisms. . A. Rev. Microbiol. 9, 227.
     [Google Scholar]
  45. Weiss, E.. ( 1965;). Adenosine triphosphate and other requirements for the utilization of glucose by agents of the psittacosis-trachoma group. . J. Bact. 90, 243.
     [Google Scholar]
  46. Weiss, E.,, Myers, W. F.,, Dressler, H. R., & Chun-Hoon, H.. ( 1964;). Glucose metabolism by agents of the psittacosis-trachoma group. . Virology 22, 551.
     [Google Scholar]
/content/journal/micro/10.1099/00221287-46-3-435
Loading
Fine Structure of Lymphogranuloma Venereum Agent and the Effects of Penicillin and 5-Fluorouracil
Microbiology 46, 435 (1967); https://doi.org/10.1099/00221287-46-3-435
/content/journal/micro/10.1099/00221287-46-3-435
/content/journal/micro/10.1099/00221287-46-3-435
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error