1887

Abstract

SUMMARY

Cultures of 10 of 19 nomenspecies of the genus liquefied a nutrient pectate gel. None of the 19 nomenspecies produced either hydrolytic polygalacturonase or pectin -eliminase. Seventeen of the nomenspecies showed a detectable, at times weak, pectinesterase activity. Seven of the 10 pectate-liquefying nomenspecies excreted polygalacturonic acid -eliminase in culture fluids containing pectin as growth substrate. The enzymo-logical basis, if any, is not at present known for the liquefaction of the nutrient pectate gel in the remaining three xanthomonads, which all lacked both the hydrolytic and the eliminative enzymes. Pectinesterase and polygalacturonic acid -eliminase were produced inducibly on pectin by most cultures which were examined; some strains of this phytopathogen formed polygalacturonic acid -eliminase constitutively on glucose, but pectinesterase was never constitutive. The polygalacturonic acid -eliminase excreted by the tested xanthomonads degraded polygalacturonic acid in a random manner. The major end-products were unsaturated di- and tri-galacturonic acids, accompanied by lesser amounts of saturated mono-, di-, and tri-galacturonic acids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-46-3-425
1967-03-01
2022-09-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/46/3/mic-46-3-425.html?itemId=/content/journal/micro/10.1099/00221287-46-3-425&mimeType=html&fmt=ahah

References

  1. Abo-El-Dahab M. K. 1964; Production of pectic and cellulolytic enzymes by Xanthomonas malvacearum. Phytopathology 54:597
    [Google Scholar]
  2. Albersheim P., Neukom H., Deuel H. 1960; Über die Bildung von ungesättigten Abbauprodukten durch ein pektinabbauendes Enzym. Helv. Chim. Acta 43:1422
    [Google Scholar]
  3. Block R., Durrum E. L., Zweig G. 1955 A Manual of Paper Chromatography and Paper Electrophoresis, 2. New York: Academic Press;
    [Google Scholar]
  4. Burkholder W. H., Starr M. P. 1948; The generic and specific characters of phytopathogenic species of Pseudomonas and Xanthomonas. Phytopathology 38:494
    [Google Scholar]
  5. Ceponis M. J., Friedman B. A. 1959; Pectolytic enzymes of Pseudomonas marginalis and their effects on lettuce. Phytopathology 49:141
    [Google Scholar]
  6. Dye D. W. 1960; Pectolytic activity in Xanthomonas. N.Z. J. Sci 3:61
    [Google Scholar]
  7. Edstrom R. D., Phaff H. J. 1964a; Purification and certain properties of pectin trans-eliminase from Aspergillus fonsecaeus. J. biol. Chem 239:2403
    [Google Scholar]
  8. Edstrom R. D., Phaff H. J. 1964b; Eliminative cleavage of pectin and of oligogalacturonide methyl esters by pectin trans-eliminase. J. biol. Chem 239:2409
    [Google Scholar]
  9. Goto M., Okabe N. 1962a; Studies on the characteristics of pectin-methylesterase excreted by Erwinia carotovora (Jones) Holland, with special reference to the comparisons of the enzymes originated from some plants. Ann. phytopathol. Soc. Japan 27:10
    [Google Scholar]
  10. Goto M., Okabe N. 1962b; Two kinds of pectin-depolymerase secreted by Erwinia carotovora (Jones) Holland. I. The enzyme production as influenced by cultural conditions and kind of isolates. Ann. phytopathol. Soc. Japan 27:231
    [Google Scholar]
  11. Kilgore W. W., Starr M. P. 1959; Catabolism of galacturonic and glucuronic acids by Erwinia carotovora. J. biol. Chem 234:2227
    [Google Scholar]
  12. Kraght A. J., Starr M. P. 1953; Pectic enzymes of Erwinia carotovora. Arch. Biochem. Biophys 42:271
    [Google Scholar]
  13. Luh B. S., Phaff H. J. 1954a; Properties of yeast polygalacturonase. Archs. Biochem. Biophys 48:23
    [Google Scholar]
  14. Luh B. S., Phaff H. J. 1954b; End products and mechanism of hydrolysis of pectin and pectic acid by yeast polygalacturonase (YPG). Archs. Biochem. Biophys 51:102
    [Google Scholar]
  15. Luh B. S., Leonard S. J., Phaff H. J. 1956; Hydrolysis of pectic materials and oligouronides by tomato polygalacturonase. Fd Res 21:448
    [Google Scholar]
  16. McColloch R. J., Kertesz Z. I. 1949; Recent developments of practical significance in the field of pectic enzymes. Fd Technol 3:94
    [Google Scholar]
  17. Nagel C. W., Vaughn R. H. 1961a; The characteristics of a polygalacturonase produced by Bacillus polymyxa. Archs. Biochem. Biophys 93:344
    [Google Scholar]
  18. Nagel C. W., Vaughn R. H. 1961b; The degradation of oligogalacturonides by the polygalacturonase of Bacillus polymyxa. Archs. Biochem. Biophys 94:328
    [Google Scholar]
  19. Nasuno S., Starr M. P. 1966a; Polygalacturonase of Erwinia carotovora. J. biol.Chem 241:5298
    [Google Scholar]
  20. Nasuno S., Starr M. P. 1966b; Pectic enzymes of Pseudomonas marginalis. Phytopathology 56:1414
    [Google Scholar]
  21. Nasuno S., Starr M. P. 1967; Polygalacturonic acid trans-eliminase of Xanthomonas campestris. Biochem. J in the press
    [Google Scholar]
  22. Ozawa J., Okamoto K. 1956; Conditions for polygalacturonase production by Erwinia species. Nogaku Kenkyu 44:81
    [Google Scholar]
  23. Roelofsen P. A. 1953; Polygalacturonase activity in yeast, Neurospora and tomato extract. Biochem. Biophys. Acta 10:410
    [Google Scholar]
  24. Sabet K. A., Dowson W. J. 1951; Action of phytopathogenic bacteria on pectate gel. Nature, Lond 168:605
    [Google Scholar]
  25. Smith W. K. 1958a; A survey of the production of pectic enzymes by plant pathogenic and other bacteria. J. gen. Microbiol 18:33
    [Google Scholar]
  26. Smith W. K. 1958b; Chromatographic examination of the products of digestion of pectic materials by culture solutions of plant pathogenic and other bacteria. J. gen. Microbiol 18:42
    [Google Scholar]
  27. Starr M. P. 1947; The causal agent of bacterial root and stem disease of guayule. Phytopathology 37:291
    [Google Scholar]
  28. Starr M. P., Moran F. 1962; Eliminative split of pectic substances by phytopathogenic soft-rot bacteria. Science 135:920
    [Google Scholar]
  29. Starr M. P., Nasuno S. 1963; Degradation of pectic substances by Xanthomonas campestris. Bact. Proc116
    [Google Scholar]
  30. Stolp H., Starr M. P., Baigent N. L. 1965; Problems in speciation of phytopathogenic pseu- domonads and xanthomonads. Ann. Rev. Phytopath 3:231
    [Google Scholar]
  31. Winstead N. N., Walker J. C. 1954; Production of vascular browning by metabolites from several pathogens. Phytopathology 44:153
    [Google Scholar]
  32. Yemm E. W. 1935; The respiration of barley plants. I. Methods for the determination of carbohydrates in leaves. Proc. R. Soc. B 117483
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-46-3-425
Loading
/content/journal/micro/10.1099/00221287-46-3-425
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error