1887

Abstract

SUMMARY

Two types of mutants of were obtained which grow anaerobically in a minimal medium with glucose as sole carbon source, but which require either succinate or methionine + lysine for aerobic growth in this medium. Only one type of mutant grew in minimal medium with succinate as sole carbon source. This type accumulated pyruvate and -oxoglutarate during growth in minimal medium with glucose and succinate. It is apparently blocked in the oxidation of -oxoacids. The other type of mutants accumulated only -oxoglutarate during growth in minimal medium with glucose and succinate. Cell-free extracts of this type of mutant did not contain -oxoglutarate dehydrogenase and washed organisms were unable to oxidize acetate. The aerobic molar growth yield of the mutant in minimal medium with glucose, methionine and lysine was much smaller than that of the wild type. Anaerobic growth of these mutants was strongly inhibited by nitrate; this inhibition was prevented by the addition of succinate.

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/content/journal/micro/10.1099/00221287-46-3-389
1967-03-01
2021-10-17
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References

  1. Back K. J. C., Westaway E. G. 1962; Studies on a mutant strain of Escherichia coli which requires both methionine and lysine for growth. J. gen. Microbiol 27:41
    [Google Scholar]
  2. Cohen G. N., Patte J. C. 1963; Some aspects of the regulation of amino acid biosynthesis in a branched pathway. Cold Spr. Harb. Symp. quant. Biol 28:513
    [Google Scholar]
  3. Davis B. D., Kornberg H. L., Nagler A., Miller P., Mingioli E. 1959; Formation and functions of succinate in Escherichia coli. Fed. Proc Fedn Am. Socs. exp. Biol 18211
    [Google Scholar]
  4. Gilvarg C. 1957; N-succinyl-l-cuaminopimelic acid, an intermediate in the biosynthesis of di-aminopimelic acid. Biochim. biophys. Act 24:216
    [Google Scholar]
  5. Haan P. G. de, Stouthamer A. H., Felix H. S., Mol A. K. 1963; Transfer of F′ from Escherichia coli k 12 to Escherichia coli b and to strains of Paracolobacter and Klebsiella. Antonie van Leeuwenhoek 29:407
    [Google Scholar]
  6. Hadjipetrou L. P. 1965; Relation between energy production and aerobic growth of bacteria. Thesis Univ. of Utrecht;
    [Google Scholar]
  7. Hadjipetrou L. P., Stouthamer A. H. 1965; Energy production during nitrate respiration by Aerobacter aerogenes. J. gen. Microbiol 38:29
    [Google Scholar]
  8. Hadjipetrou L. P., Gerrits J. P., Teulings F. A. G., Stouthamer A. H. 1964; Relation between energy production and growth of Aerobacter aerogenes. J. gen. Microbiol 36:139
    [Google Scholar]
  9. Hager L. P., Kornberg H. L. 1961; On the mechanism of α-oxoglutaratedehydrogenase in Escherichia coli. Biochem. J 78:194
    [Google Scholar]
  10. Lascelles J. 1962; Tetrapyrrole synthesis in microorganisms. In The Bacteria Gunsalus I. C., Stanier R. Y. 3335 New York: Academic Press;
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  12. Pichinoty F., Mottet F., Bigliardi-Rouvier V. J., Forget P. 1963; Le cycle de Krebs chez les bactéries denitrifiantes. Z. Naturforschg 186:492
    [Google Scholar]
  13. Reekers A. 1964; On the production of α-ketoglutaric acid by Pseudomonas reptilovora, Coldwell and Ryerson. Thesis University of Delft;
    [Google Scholar]
  14. Rowbury R. J., Woods D. D. 1964; O-succinyl-homoserine as an intermediate in the synthesis of cystathionine by Escherichia coli. J. gen. Microbiol 36:341
    [Google Scholar]
  15. Sanadi D. R., Littlefield J. W., Bock R. M. 1952; Studies on α-ketoglutaric oxidase. II. Purification and properties. J. biol. Chem 197:851
    [Google Scholar]
  16. Stouthamer A. H., Haan P. G. de, Nijkamp H. J. J. 1965; Mapping of purine markers in Escherichia coli k12. Genet. Res. Comb 6:442
    [Google Scholar]
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