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Host cell reactivation (hcr) of phage T1 by two strains of Escherichia coli k 12, Hfr c (λ +)thy – and Hfr b 1 (λ –)thy –, was studied. Strain Hfr c showed decreased ability to reactivate ultraviolet (u.v.)-irradiated phage T1, as predicted from its behaviour in mating experiments after thymine starvation. Thymine starvation of host Hfr B 1 bacteria resembled u.v. irradiation in decreasing hcr, but thymine starvation of Hfrc increased hcr, apparently as a result of λ induction. The presence of λ prophage in E. coli k 12 was found to decrease hcr.
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