1887

Abstract

SUMMARY

A simple chemically defined medium for examining the motility of 12 was designed. The essential components were: (1) a chelating agent to protect the motility against inhibition by traces of heavy metal ions; (2) a buffer to keep the pH value at the optimum between pH 6·0 and 7·5; (3) an energy source to stimulate the motility above that allowed by an endogenous energy source. Oxygen was required unless an energy source was provided which yielded energy anaerobically. A temperature optimum was determined.

A chemically defined growth medium capable of producing motile bacteria was devised. It was found that the presence of glucose or growth above 37° prevented synthesis of flagella.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-46-2-175
1967-02-01
2022-12-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/46/2/mic-46-2-175.html?itemId=/content/journal/micro/10.1099/00221287-46-2-175&mimeType=html&fmt=ahah

References

  1. Adler J. 1966; The effect of amino acids and oxygen on chemotaxis in Escherichia coli. J. Bact 92:121
    [Google Scholar]
  2. Adler J., Dahl M. M. 1967; A method for measuring the motility of bacteria and for comparing random and non-random motility. J. gen. Microbiol 46:161
    [Google Scholar]
  3. Greenberg D. M. 1951 Amino acids and Proteins465 Springfield, Illinois: C. C. Thomas;
    [Google Scholar]
  4. Kerridge D. 1959; Synthesis of flagella by amino acid-requiring mutants of Salmonella typhimurium. J. gen. Microbiol 21:168
    [Google Scholar]
  5. Leifson E. 1951; Staining, shape, and arrangement of bacterial flagella. J. Bact 62:377
    [Google Scholar]
  6. Magasanik B. 1961; Catabolite repression. Cold Spr. Harb. Symp. quant. Biol 26:249
    [Google Scholar]
  7. Martell A. E., Calvin M. 1952 Chemistry of the Metal Chelate Compounds New York: Prentice-Hall, Inc;
    [Google Scholar]
  8. Meynell E. W. 1961; A phage, Φχ, which attacks motile bacteria. J. gen. Microbiol 25:253
    [Google Scholar]
  9. Ogiuti K. 1936; Untersuchungen über die Geschwindigkeit der Eigenbewegung von Bakterien. Jap. J. exp. Med 14:19
    [Google Scholar]
  10. Pardee A. B., Prestidge L. S. 1955; Induced formation of serine and threonine deaminases by Escherichia coli. J. Bact 70:667
    [Google Scholar]
  11. Quadling C., Stocker B. A. D. 1962; An environmentally induced transition from the flagellated to the non-flagellated state in Salmonella typhimurium: the fate of parental flagella at cell division. J. gen. Microbiol 28:257
    [Google Scholar]
  12. Rothschild Lord, Tyler A. 1954; The physiology of sea-urchin spermatozoa; action of versene. J. exp. Biol 31:252
    [Google Scholar]
  13. Sherris J. C., Preston N. W., Shoesmith J. G. 1957; The influence of oxygen and arginine on the motility of a strain of Pseudomonas sp. J. gen. Microbiol 16:86
    [Google Scholar]
  14. Stocker B. A. D., Campbell J. C. 1959; The effect of non-lethal deflagellation on bacterial motility and observations on flagellar regeneration. J. gen. Microbiol 20:670
    [Google Scholar]
  15. Tyler A. 1953; Prolongation of life-span of sea urchin spermatozoa. Biol. Bull. mar. biol. Lab., Woods Hole 104:224
    [Google Scholar]
  16. Umbarger H. E., Brown B. 1957; Threonine deamination in Escherichia coli. II. Evidence for two l-threonine deaminases. J. Bact 73:105
    [Google Scholar]
  17. Weibull C. 1948; Some chemical and physico-chemical properties of the flagella of Proteus vulgaris. Biochim. biophys. Acta 2:351
    [Google Scholar]
  18. Weibull C. 1960; Movement. In The Bacteria Gunsalus I. C., Stanier R. Y. i153 New York: Academic Press;
    [Google Scholar]
  19. Wood W. A., Gunsalus I. C. 1949; Serine and threonine deaminases of Escherichia coli: activators for a cell-free enzyme. J. biol. Chem 181:171
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-46-2-175
Loading
/content/journal/micro/10.1099/00221287-46-2-175
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error