SUMMARY: The ability of suspensions of washed spores of to oxidize triglycerides was markedly stimulated by L-proline, L-alanine and L-serine, although all the sources of nitrogen tested were effective over extended periods to different degrees. The rate of oxidation was further increased by the addition of certain sugars, although these were without effect in the absence of a nitrogen source. The same compounds that stimulated methylketone formation from triglycerides also promoted the rapid germination of spores, suggesting that common reactions were involved. The maximum yield of methylketone obtained was 25% from trioctanoin and trihexanoin at pH 6·0, with lower yields from tridecanoin, tributyrin and trilaurin. The addition of sodium azide, and to a lesser extent of 2,4-dinitrophenol and certain organophosphours compounds, inhibited the oxidation of triglycerides. The possible role of fungal esterases in spore germination is discussed.


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