@article{mbs:/content/journal/micro/10.1099/00221287-46-1-143, author = "Bernheimer, A. W. and Grushoff, Phyllis", title = "Cereolysin : Production, Purification and Partial Characterization", journal= "Microbiology", year = "1967", volume = "46", number = "1", pages = "143-150", doi = "https://doi.org/10.1099/00221287-46-1-143", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-46-1-143", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY A method is described for production of the extracellular haemolytic growth-product of Bacillus cereus. The lytic substance, called ‘cereolysin’, can be purified by fractional precipitation with ammonium sulphate, density-gradient electrophoresis and gel filtration. The purified material is a labile protein of molecular weight about 52,000. It has a very high order of haemolytic activity, is lethal for mice, and resembles streptolysin O in being inhibited by cholesterol, but differs from streptolysin O in other respects.", }