Inhibition of Carotenoid Synthesis in a Mutant of Free

Abstract

SUMMARY: mutant 5·6 which produces phytoene, β- and γ-carotene, neolycopene A, lycopene and neurosporaxanthin was subjected to the effect of the following inhibitors of carotenoid synthesis: diphenylamine, 2-hydroxydiphenyl, β-ionone, methylheptenone. Certain intermediates in the Porter-Anderson (1962) pathway for carotenoid biosynthesis not previously present were then observed, namely: phytofluene, β-zeacarotene, ζ-carotene, neurosporene. When the mutant was grown in the presence of diphenylamine, washed free from it and then grown again in 1 % glucose in /15 KHPO, the more unsaturated polyenes seem to be formed at the expense of the more saturated ones. There was also evidence that γ-carotene was formed via β-zeacarotene and the sequence of carotenoid formation seems to agree very closely to that suggested by Porter & Anderson (1962).

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/content/journal/micro/10.1099/00221287-45-3-531
1966-12-01
2024-03-29
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References

  1. Chichester C. O., Wong P. S., Mackinney G. 1954; On the biosynthesis of Carotenoids.. Plant Physiol 29:238
    [Google Scholar]
  2. Davies B. H. 1961; The biosynthesis of carotenoids of Rhizophlyctis rosea.. Biochem. J 80:48
    [Google Scholar]
  3. Davies B. H., Villoutreix J., Williams R. J. H., Goodwin T. W. 1963; The possible role of β-zeacarotene in carotenoid synthesis.. Biochem. J 89:96
    [Google Scholar]
  4. Goodwin T. W. 1952; Identification of the minor polyene components of the fungus Phycomyces blakesleeanus and a study of their synthesis under various cultural conditions.. Biochem. J 50:550
    [Google Scholar]
  5. Goodwin T. W. 1953; The biogenesis of carotenoids.. J. Sci. Fd Agric 5:209
    [Google Scholar]
  6. Goodwin T. W., Osman H. G. 1953; General cultural conditions controlling carotenoid (spirilloxanthin) synthesis in the photosynthetic bacterium Rhodospirillum rubrum.. Biochem. J 53:541
    [Google Scholar]
  7. Goodwin T. W., Jamikorn M., Willmer J. S. 1953; Further observations concerning the action of diphenylamine in inhibiting the synthesis of β-carotene in Phycomyces blakesleeanus . Biochem. J 53:531
    [Google Scholar]
  8. Haxo F. 1949; Studies on the carotenoid pigments of Neurospora.. Arch. Biochem 20:400
    [Google Scholar]
  9. Heale J. B., Isaac I. 1964; Dark pigment formation in Verticillium albo-atrum.. Nature, Lond 202:412
    [Google Scholar]
  10. Krzeminski L. F., Quackenbush F. W. 1960; Incorporation of C14-labelled compounds into carotenes by Neurospora crassa.. Arch. Biochem. Biophys 88:287
    [Google Scholar]
  11. Mackinney G., Nakayama T., Buss C. D., Chichester C. O. 1952; Carotenoid production in Phycomyces.. J. Am. chem. Soc 74:3456
    [Google Scholar]
  12. Nakayama T., Chichester C. O., Lukton A., Mackinney G. 1957; Phytoene production in Phycomyces.. Arch. Biochem. Biophys 66:310
    [Google Scholar]
  13. Olson J. A., Knizley J. H. 1962; The effect of diphenylamine on carotenoid, sterol and fatty acid synthesis in Phycomyces blakesleeanus.. Arch. Biochem. Biophys 97:138
    [Google Scholar]
  14. Porter J. W., Anderson D. G. 1962; The biosynthesis of carotenes.. Arch. Biochem. Biophys 97:520
    [Google Scholar]
  15. Porter J. W., Lincoln R. E. 1950; The mechanism of carotene biosynthesis.. Arch. Biochem 27:390
    [Google Scholar]
  16. Rilling H. C. 1965; A study of inhibition of carotenoid synthesis.. Arch. Biochem. Biophys 110:39
    [Google Scholar]
  17. Rüegg R., Schwieter U., Ryser G., Schudel P., Isler O. 1961; Synthesen in der Carotenoid-Reihe.. Helv. chim. Acta 44:994
    [Google Scholar]
  18. Simpson K. L., Nakayama T. O. M., Chichester C. O. 1964; Biosynthesis of yeast carotenoids.. J. Bact 88:1688
    [Google Scholar]
  19. Slechta L., Gabriel O., Ostenhof O. 1958; Separation of carotenoid synthesis from fat synthesis in Rhodotorula gracilis by diphenylamine.. Nature, Lond 181:268
    [Google Scholar]
  20. Turian G. 1957; Recherches sur l’action anticaroténogène de la diphénylamine et ses consequences sur la morphogenèse reproductive chez Allomyces et Neurospora.. Physiol. Plant 10:667
    [Google Scholar]
  21. Turian G., Haxo F. 1954; Minor polyene components in the sexual phase of Allomyces javanicus.. Bot. Gaz 115:254
    [Google Scholar]
  22. Valadon L. R. G., Heale J. B. 1965; Carotenoids in mutants of Verticillium alboatrum.. J. gen. microbiol 41:341
    [Google Scholar]
  23. Villoutreix J. 1960 Sur les Caroténoïdes d’une Levure Rouge: Rhodotorula mucilaginosa (Jorg) Harrison. Nancy: Imprimerie Grandville.;
    [Google Scholar]
  24. Zalokar M. 1954; Studies on biosynthesis of carotenoids in Neurospora crassa.. Arch. Biochem. Biophys 50:71
    [Google Scholar]
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