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Regeneration of Mycelial Protoplasts of Fusarium culmorum
Abstract
SUMMARY: The process of regeneration of Fusarium culmorum protoplasts, obtained by means of strepzyme RA, has been followed. Protoplast regeneration started with the formation of cellular aggregates originating a mass of globular forms which later gave rise to the formation of a pseudomycelial form. Regeneration of protoplasts was formed in three different ways: (a) by means of a chain of yeast-like forms and later originating a germ tube; (b) by direct formation of the germ tube from the protoplast; (c) through a complicated process with the formation of various globular forms and giving rise to the formation of the germ tube at the end. Maximum regeneration, about 80 % of the protoplasts, was found in the presence of 2% (w/v) sucrose (or 1% (w/v) glucose + 1% (w/v) fructose) + 10% (w/v) sorbose + 0·2% agar + the mineral salts of the Czapek medium. No other sugars were able to substitute for sucrose and sorbose. Agar was the best substance for regeneration; gelatin produced an inhibition. Regeneration was greater under acid conditions, alkaline pH values interfering with the phenomenon. The number of nuclei/protoplast varied from 1 to 4, the lack of them in some spherical forms perhaps being the cause of failure to regenerate. No differences were found in the regenerative process as between protoplasts obtained by the use of snail or by microbial lytic enzymes.
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© Society for General Microbiology 1966