SUMMARY: Virions of the papillomaviruses of man, cattle, dog and rabbit were compared by immunodiffusion in agar. Antigen reactants were prepared from saline extracts of warts, and consisted either of crude virus-particle concentrates, or of ‘full’ or ‘empty’ fractions from equilibrium density gradients. There were no cross-reactions between the wart viruses of different species. Within any one species, ‘full’ and ‘empty’ particles gave antigenically identical single bands of precipitate, whether derived from the same or from different warts; but some old preparations of ‘empty’ particles gave a second band.


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