SUMMARY: Two Hfr strains of 12 were mated with stationary phase 677 F at intervals during thymine starvation. With both strains, Hfr bacteria retained their capability of chromosome transfer for a period after loss of colony-forming ability. Eventual loss of transfer capability in strain HfrC was associated with inactivation of some early stage of the transfer process. Thymine starved HfrC transferred the genetic markers and with a normal gradient but HfrB1 showed marker inactivation. The extent of marker inactivation in this strain was consistent with the presence of 10-20 breaks/chromosome. It is suggested that the strains may have behaved differently in these experiments as a result of differences in their ability to modify primary genetic lesions resulting from thymine starvation.


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