1887

Abstract

SUMMARY: Two Hfr strains of 12 were mated with stationary phase 677 F at intervals during thymine starvation. With both strains, Hfr bacteria retained their capability of chromosome transfer for a period after loss of colony-forming ability. Eventual loss of transfer capability in strain HfrC was associated with inactivation of some early stage of the transfer process. Thymine starved HfrC transferred the genetic markers and with a normal gradient but HfrB1 showed marker inactivation. The extent of marker inactivation in this strain was consistent with the presence of 10-20 breaks/chromosome. It is suggested that the strains may have behaved differently in these experiments as a result of differences in their ability to modify primary genetic lesions resulting from thymine starvation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-45-3-489
1966-12-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/45/3/mic-45-3-489.html?itemId=/content/journal/micro/10.1099/00221287-45-3-489&mimeType=html&fmt=ahah

References

  1. Cohen S. S., Barner H. D. 1954; Studies on unbalanced growth in Escherichia coli.. Proc. natn. Acad. Sci. U.S.A. 40:885
    [Google Scholar]
  2. Coughlin C. A., Adelberg E. A. 1956; Bacterial mutation induced by thymine starvation.. Nature, Lond 178:531
    [Google Scholar]
  3. Davison P. F., Freifelder D., Holloway B. W. 1964; Interruptions in the polynucleotide strands of bacteriophage DNA.. J. mol. Biol 8:1
    [Google Scholar]
  4. Freifelder J. D., e O. 1964; Energy requirement for thymineless death in cells of Escherichia coli.. J. Bact 88:987
    [Google Scholar]
  5. Fuerst C. R., Stent G. S. 1956; Inactivation of bacteria by decay of incorporated radioactive phosphorus.. J. gen. Physiol 40:73
    [Google Scholar]
  6. Gallant J., Spottswood T. 1965; The recombinogenic effect of thymidylate starvation in Escherichia coli merodiploids.. Genetics 52:107
    [Google Scholar]
  7. Gallant J., Suskind S. R. 1961; Relationship between thymineless death and ultraviolet inactivation in Escherichia coli.. J. Bact 82:187
    [Google Scholar]
  8. Gallant J., Suskind S. R. 1962; R.N.A. synthesis and thymineless death.. Biochim. biophys. Acta 55:627
    [Google Scholar]
  9. Gross J. D. 1963; The effect of unbalanced growth on recombinant formation in Escherichia coli.. Genet. Res 4:457
    [Google Scholar]
  10. DeHaan P. G., Gross J. D. 1962; Transfer delay and chromosome withdrawal during conjugation in Escherichia coli.. Genet. Res 3:251
    [Google Scholar]
  11. Jacob F., Wollman E. L. 1958; Genetical and physical determinations of chromosomal segments in Escherichia coli.. Symp. Soc. exp. Biol 12:75
    [Google Scholar]
  12. Jacob F., Wollman E. L. 1961 Sexuality and The Genetics of Bacteria137 New York: Academic Press.;
    [Google Scholar]
  13. Kanazir D. 1958; Apparent mutagenicity of thymine deficiency.. Biochim. biophys. Acta 30:20
    [Google Scholar]
  14. Korn D., Weissbach A. 1962; Thymineless induction in Escherichia coli k12 (A).. Biochim. biophys. Acta 61:775
    [Google Scholar]
  15. Luzatti D., Revel C. 1962; Effet léthal de la carence en thymine: état de l’acide deoxyribonucléique au cours de cette carence.. Biochim. biophys. Acta 61:305
    [Google Scholar]
  16. Maaløe O. 1961; The control of normal DNA replication in bacteria.. Cold Spring Harb. Symp. quant. Biol 26:45
    [Google Scholar]
  17. Melechen N. E., Skaar P. D. 1962; The provocation of an early step of induction by thymine deprivation.. Virology 16:21
    [Google Scholar]
  18. Mennigmann H., Sybalski W. 1962; Molecular mechanism of thymineless death.. Biochem. biophys. Res. Comm 9:398
    [Google Scholar]
  19. Okada T., Ryan F. J. 1961; A method for securing thymineless mutants of Escherichia coli.. Z.-Vererbungslehre 92:403
    [Google Scholar]
  20. Pritchard R. H. 1965; The relationship between conjugation, recombination and DNA synthesis in Escherichia coli . 10th Int. Congress Genetics. Genetics Today 2:56
    [Google Scholar]
  21. Rasmussen R. E., Painter R. B. 1963; On the early onset of thymineless death occurring after exposure to ultra-violet light.. Biochim. biophys. Acta 76:157
    [Google Scholar]
  22. Setlow R. B., Carrier W. L. 1964; The disappearance of thymine dimers from DNA: an error correcting mechanism.. Proc. natn. Acad. Sci. U.S.A 51:226
    [Google Scholar]
  23. Sicard N., Anagnostopoulos C. 1964; Activité transformante de l’acide desoxy-ribonucléique de Bacillus subtilis lors de la carence en thymine.. C. r. Acad. Sci. Paris 259:4173
    [Google Scholar]
  24. Sicard N., Devoret R. 1962; Effets de la carence en thymine sur les souches d’Escherichia Coli lysogènes k12t et colicinogène 15t . C. r. Acad. Sci. Paris 255:1417
    [Google Scholar]
  25. Stacey K. A., Symonds N. D., Atkinson C. 1966 (In Press.)
  26. Weinberg R., Latham A. B. 1956; Apparent mutagenic effect of thymine deficiency for a thymine requiring strain of E. coli.. J. Bact 72:570
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-45-3-489
Loading
/content/journal/micro/10.1099/00221287-45-3-489
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error