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SUMMARY: The reduction of ferricyanide by resting and actively growing Escherichia coli was studied. Under anaerobic conditions ferricyanide acted as a hydrogen acceptor for the complete oxidation of glucose and TCA cycle intermediates. However, reduction of ferricyanide was not coupled to ATP formation. The anaerobic molar growth yield from glucose in the presence of ferricyanide was even less than in its absence. Ferricyanide repressed the synthesis of formate hydrogenylase.
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