Comparative Studies on the Agglutination of Fowl Red Blood Cells by the Haemagglutinins of Vaccinia and Coxsackie A 7 Viruses Free

Abstract

SUMMARY: Red blood cells from a group of fowls were sensitive to the haemag-glutinins of vaccinia and Coxsackie A7 viruses but the red cells from certain fowls exhibited differences in their relative sensitivities. Differences between the reaction of these haemagglutinins with susceptible red cells of the same sensitivity were shown by treatment with chemical and physical agents. There were differences in the effect of pH value on the haemagglutination titres and, unlike Coxsackie A7 hemagglutinin, the adsorption of vaccinia haemagglutinin to susceptible red cells was inhibited by divalent cations. Red cell receptors for both haemagglutinins were insensitive to RDE (receptor destroying enzyme) but were inactivated by treatment with potassium periodate, papain or α-chymotrypsin. There were quantitative differences in the degrees or rates of receptor destruction by these reagents. Haemagglutination by vaccinia and Coxsackie A7 haemagglutinins was inhibited only by homologous antiserum. These qualitative and quantitative differences indicate separate red cell receptors for the two haemagglutinins.

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1966-12-01
2024-03-29
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References

  1. Andrewes C. H., Bang F. B., Burnet F. M. 1955; A short description of the myxo-virus group (influenza and related viruses).. Virology 1:176
    [Google Scholar]
  2. Briody A. 1951; A ‘Unitarian’ view of vaccinia haemagglutinin.. Yale J. Biol. Med 24:1
    [Google Scholar]
  3. Buckland F. E., Tyrell D. A. J. 1963; A comparative study of virus haemagglutinins. The stability of haemagglutinins and red cell receptors to certain chemical and physical treatments.. J. gen. Microbiol 32:241
    [Google Scholar]
  4. Burnet F. M., Boake W. C. 1946; The relationship between the virus of infectious ectromelia of mice and vaccinia virus.. J. Immunol 53:1
    [Google Scholar]
  5. Burnet F. M., Stone J. D. 1946; The haemagglutinins of vaccinia and ectromelia viruses.. Aust. J. exp. Biol. med. Sci 24:1
    [Google Scholar]
  6. Burnet F. M., Stone J. D. 1947; The receptor destroying enzyme of V. cholerae.. Aust. J. exp. Biol. med. Sci 25:227
    [Google Scholar]
  7. Chu C. M. 1948; Studies on vaccinia haemagglutinin: some physicochemical properties.. J. Hyg., Camb 46:42
    [Google Scholar]
  8. Clark E., Nagler F. P. O. 1943; Haemagglutination by viruses. The range of susceptible cells with special reference to agglutination by vaccinia virus.. Aust. J. exp. Biol. med. Sci 21:103
    [Google Scholar]
  9. Gogolak F. M., Ross M. R. 1955; The properties and chemical nature of psittacosis virus haemagglutinin.. Virology 1:176
    [Google Scholar]
  10. Grist N. R. 1960; Isolation of Coxsackie A7 virus in Scotland.. Lancet i:1054
    [Google Scholar]
  11. Klenk E., Lempfrid H. 1957; Über die Natur der Zellreceptoren für das Influenza virus.. Z. Physiol. Chem 307:278
    [Google Scholar]
  12. McCarthy K., Helbert D. 1960; A comparison of the haemagglutinins of variola, alastrim, vaccinia, cowpox and ectromelia viruses.. J. Path. Bact 79:416
    [Google Scholar]
  13. Neff B. J., Ackermann W. W., Preston R. E. 1965; Studies of vaccinia haemagglutinin obtained from vaccinia infected tissue. Density gradient centrifugation and electron microscopy.. Proc. Soc. exp. Biol. Med 118:664
    [Google Scholar]
  14. Philipson L., Bengtsson S., Brishammar S., Svennerholm L., Zetterqvist O. 1964; Purification and chemical analysis of the erythrocyte receptor for haemagglutinating enteroviruses.. Virology 22:580
    [Google Scholar]
  15. Stone J. D. 1946a; Inactivation of vaccinia and ectromelia virus by lecithinase.. Aust. J. exp. Biol. med. Sci 24:191
    [Google Scholar]
  16. Stone J. D. 1946b; Lipid haemagglutinins.. Aust. J. exp. Biol. med. Sci 24:197
    [Google Scholar]
  17. Williamson J. D., Grist N. R. 1965; Studies on the haemagglutinin present in Coxsackie A7 virus-infected suckling mouse tissue.. J. gen. Microbiol 41:283
    [Google Scholar]
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