1887

Abstract

SUMMARY: Cholesterol enrichment cultures from soil samples yielded various and spp. capable of metabolizing cholesterol aerobically as a sole carbon source. One of these isolates, designated 148, formed haloes (cholesterol-free zones surrounding colonies) on cholesterol mineral salts agar. This organism was selected for further study as it was able to utilize 80-100% of 0·1% (w/v) cholesterol in a mineral salts medium in 6 days. Oxidation of the sterol was initiated by a cholesterol dehydrogenase, giving 4-cholestene-3-one. The latter compound was then hydroxylated to form 4-cholestene-4-ol-3-one. Isotopic tracer studies revealed all of carbon-4 and most of carbon-26 of cholesterol were converted to C0 However, some of carbon-26 was converted to cell material.

148 gave several variants, one of which ( 148 no. 2 var. A) could be reclassified as a Nocardia, using the same method of Gordon & Smith (1955).

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/content/journal/micro/10.1099/00221287-45-3-441
1966-12-01
2024-04-24
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References

  1. Arnaudi C. 1951; Oxydations du microbiennes des steroids.. Experientia 5:120
    [Google Scholar]
  2. Arnaudi C. 1951; Research on the microbiological degradation of steroids.. Appl. Microbiol 2:274
    [Google Scholar]
  3. Bennet R. D., Heftman E. 1962; Thin layer chromatography of corticosteroids.. J. Chromatog 9:348
    [Google Scholar]
  4. Davis J. R., Brown R. L., Lewis H. L., Peterson G. E. 1964; Cholesterol utilization by Streptomyces spp.. Bact Proc100
    [Google Scholar]
  5. Fieser L. G., Stevenson R. 1954; Cholesterol and companions. Oxidation of 5- cholestene-3-one with lead tetraacetate.. J. Am. chem. Soc 76:1731
    [Google Scholar]
  6. Gordon R. E., Smith M. M. 1955; A proposed group of characters for the separation of Streptomyces and Nocardia.. J. Bact 69:147
    [Google Scholar]
  7. Haag F. 1927; Die saphrophytisehen Myeobaeterien.. Zentbl. Bakt. ParasitKde Abt. II 71:1
    [Google Scholar]
  8. Kramli A., Horvath J. 1947; Microbiological oxidation of cholesterol with Azotobacter.. Nature, Lond 160:639
    [Google Scholar]
  9. Lewis H. L. 1962; Evidence for cholesterol metabolism by soil streptomycetes.. M.S. Thesis: University of Houston, Texas.;
    [Google Scholar]
  10. Peterson G. E., Lewis H. L., Davis J. R. 1962; Preparation of uniform dispersions of cholesterol and other water insoluble carbon sources in agar media.. J. lipid. Res 3:275
    [Google Scholar]
  11. Peterson G. E., Mandy W. J., Futch H., Luckey D. 1962; Cholesterol deposition in Penicillium spp.. Can. J. Microbiol 8:193
    [Google Scholar]
  12. Randerath K. 1963 Thin Layer Chromatography New York: Academic Press.;
    [Google Scholar]
  13. Schatz A., Savard K., Pintner I. 1949; The ability of soil microorganisms to decompose cholesterol.. J. Bact 58:117
    [Google Scholar]
  14. Söhngen N. L. 1913; Benzin, Petroleum, Paraffinöl and Paraffin als Kohlenstoff-und Energiequelle für Mikroben.. Zentbl. Bakt. ParasitKde (Abt. II) 37:595
    [Google Scholar]
  15. Sperry W. M., Webb M. 1950; A revision of the Schoenheimer–Sperry method for cholesterol determination.. J. biol. Chem 187:97
    [Google Scholar]
  16. Stadtman T. C., Cherkes A., Anfinson C. B. 1954; Studies on the microbiological degradation of cholesterol.. J. biol. Chem 206:511
    [Google Scholar]
  17. Tak J. D. 1942; On bacteria decomposing cholesterol.. Antonie van Leeuwenhoek 8:32
    [Google Scholar]
  18. Turfitt G. E. 1943; The microbiological degradation of steroids. I. The sterol content of soils.. Biochem. J 37:115
    [Google Scholar]
  19. Turfitt G. E. 1944; The microbiological degradation of steroids. II. Oxidation of cholesterol by Proactinomyces spp.. Biochem. J 38:492
    [Google Scholar]
  20. Turfitt G. E. 1948; Microbiological agencies in the degradation of steroids. II. Steroid utilization by the microflora of soils.. J. Bact 54:557
    [Google Scholar]
  21. Whitmarsh J. M. 1964; Intermediates of microbiological metabolism of cholesterol.. Biochem. J 90:23
    [Google Scholar]
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