1887

Abstract

SUMMARY: ‘Lucerne’ auxotrophs of produced by ultraviolet irradiation and derived from the same parental strain formed heterokaryons on minimal agar 4-5 weeks after inoculation. A degree of heterokaryon incompatibility was shown between auxotrophs derived from different isolates of and one of . Conidial methods of heterokaryon synthesis failed but transference of agar inocula from complete medium agar cultures to minimal medium agar was successful. Conidia were uni-nucleate and nuclear migration subsequent to germ-tube fusion was observed. Heterokaryons grew slowly and were unstable, but by using di-auxotrophs a greater stability was achieved and a number were subcultured successfully for more than a year. Spore analysis yielded colonies of both parental types with various ratios. A heterokaryon test between a hyaline and normal dark auxotroph of indicated that an important system controlling morphogenesis might be linked with a cytoplasmic factor. A hypothesis is proposed to account for the production and degree of stability of the hyaéine variants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-45-3-419
1966-12-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/45/3/mic-45-3-419.html?itemId=/content/journal/micro/10.1099/00221287-45-3-419&mimeType=html&fmt=ahah

References

  1. Van Beyma Thoe Kingma F. H. II. 1939; Über einige Formen von Verticillium dahliae Klebahn.. Antonie van Leeuwenhoek 6:33
    [Google Scholar]
  2. Brandt W. H. 1962; Manganese stimulation of melanin synthesis and mierosclerotia development in Verticillium.. Plant Physiol 37:(Suppl.)30
    [Google Scholar]
  3. Brandt W. H. 1964; Morphogenesis in Verticillium: Effects of light and ultra violet radiation on mierosclerotia and melanin.. Can. J. Bot 42:1017
    [Google Scholar]
  4. Brandt W. H., Roth J. N. 1965; Loss of melanin-containing structures by Verticillium cultures.. Phytopathology 55:1200
    [Google Scholar]
  5. Caroselli N. E. 1957; Verticillium wilt of maple.. Bull. R. I. agric. Exp. Sta. no. 3351
    [Google Scholar]
  6. Hastie A. C. 1962; Genetic recombination in the hop-wilt fungus Verticillium albo-atrum.. J. gen. Microbiol 27:373
    [Google Scholar]
  7. Hastie A. C. 1964; The parasexual cycle in Verticillium albo-atrum.. Genet. Res 5:305
    [Google Scholar]
  8. Heale J. B., Isaac I. 1963; Wilt of lucerne caused by species of Verticillium. IV. Pathogenicity of V. albo-atrum and V. dahliae to lucerne and other crops; spread and survival of V. albo-atrum in soil and in weeds; effect upon lucerne production.. Ann. appl. Biol 52:439
    [Google Scholar]
  9. Heale J. B., Isaac I. 1964; Dark pigment formation in Verticillium albo-atrum.. Nature, Lond 202:412
    [Google Scholar]
  10. Heale J. B., Isaac I. 1965; Environmental factors in the production of dark resting structures in Verticillium albo-atrum V. dahliae and V. tricorpus.. Trans. Br. my col. Soc 48:39
    [Google Scholar]
  11. Kaiser W. J. 1964; Effects of light on growth and sporulation of the Verticillium wilt fungus.. Phytopathology 54:765
    [Google Scholar]
  12. Nelson R. 1950; Verticillium wilt of peppermint.. Tech. Bull. Mich. (St. Coll.) agric. Exp. Stn. no. 2211
    [Google Scholar]
  13. Parmeter J. R., Snyder W. C., Reichle R. E. 1963; Heterokaryosis and variability in plant-pathogenic fungi.. A. Rev. Phytopathology 1:51
    [Google Scholar]
  14. Petiiybridge G. H. 1916; The Verticillium disease of the potato.. Scient. Proc. R. Dubl. Soc 15:63
    [Google Scholar]
  15. Reinke J., Bertiiold G. 1879; Die Zersetzung der Kartoffel durch Pilze.. Enters. Bot. Lab. Univ. Gottingen Berlin: Verl. van Wiegandt, Hempel & Pavey.;
    [Google Scholar]
  16. Robinson D. B., Larson R., Walker J. C. 1957; Verticillium wilt of potato.. Res. Bull, agric. Exp. Sta. Univ. Wis. no. 2021
    [Google Scholar]
  17. Rudolph B. A. 1931; Verticillium hadromycosis.. Ililgardia 5:197
    [Google Scholar]
  18. Thomas M. 1955; Melanins.. Modern Methods of Plant Analysis 4:661
    [Google Scholar]
  19. Valadon L. R. G., Heale J. B. 1964; Carotenoid pigments in a mutant strain of Verticillium albo-atrum . Reinke & Berthold. Nature, Lond 202:512
    [Google Scholar]
  20. Valadon L. R. G., Heale J. B. 1965; Carotenoids in mutants of Verticillium albo-atrum.. J. gen. Microbiol 41:341
    [Google Scholar]
  21. Waggoner P. E. 1956; Variation in Verticillium albo-atrum from potato.. Plant Dis. Reptr 40:429
    [Google Scholar]
/content/journal/micro/10.1099/00221287-45-3-419
Loading
/content/journal/micro/10.1099/00221287-45-3-419
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error