SUMMARY: ‘Lucerne’ auxotrophs of produced by ultraviolet irradiation and derived from the same parental strain formed heterokaryons on minimal agar 4-5 weeks after inoculation. A degree of heterokaryon incompatibility was shown between auxotrophs derived from different isolates of and one of . Conidial methods of heterokaryon synthesis failed but transference of agar inocula from complete medium agar cultures to minimal medium agar was successful. Conidia were uni-nucleate and nuclear migration subsequent to germ-tube fusion was observed. Heterokaryons grew slowly and were unstable, but by using di-auxotrophs a greater stability was achieved and a number were subcultured successfully for more than a year. Spore analysis yielded colonies of both parental types with various ratios. A heterokaryon test between a hyaline and normal dark auxotroph of indicated that an important system controlling morphogenesis might be linked with a cytoplasmic factor. A hypothesis is proposed to account for the production and degree of stability of the hyaéine variants.


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