SUMMARY: ‘Lucerne’ auxotrophs of produced by ultraviolet irradiation and derived from the same parental strain formed heterokaryons on minimal agar 4-5 weeks after inoculation. A degree of heterokaryon incompatibility was shown between auxotrophs derived from different isolates of and one of . Conidial methods of heterokaryon synthesis failed but transference of agar inocula from complete medium agar cultures to minimal medium agar was successful. Conidia were uni-nucleate and nuclear migration subsequent to germ-tube fusion was observed. Heterokaryons grew slowly and were unstable, but by using di-auxotrophs a greater stability was achieved and a number were subcultured successfully for more than a year. Spore analysis yielded colonies of both parental types with various ratios. A heterokaryon test between a hyaline and normal dark auxotroph of indicated that an important system controlling morphogenesis might be linked with a cytoplasmic factor. A hypothesis is proposed to account for the production and degree of stability of the hyaéine variants.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error