1887

Abstract

SUMMARY: Caulobacter bacteriophages containing ribonucleic acid have been found to attach to pili of their hosts. Only when the phage is actually lytic for a given host can the phage+pilus complexes be observed; non-specific complexes are not formed. Removal of the pili from the host by shear treatment before phage adsorption resulted in effective inhibition of phage adsorption. While swarmer caulobacters frequently possessed pili which were located at the flagellated pole of the organism, stalked caulobacters were almost always devoid of these structures. Adsorption experiments with segregated populations of caulobacters indicated that stalked caulobacters adsorbed RNA phages very poorly, while in swarmer populations and unsegregated cultures the RNA phages were efficiently adsorbed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-45-2-347
1966-11-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/45/2/mic-45-2-347.html?itemId=/content/journal/micro/10.1099/00221287-45-2-347&mimeType=html&fmt=ahah

References

  1. Adams M. 1959 Bacteriophages New York: Interscience Publishers, Inc.;
    [Google Scholar]
  2. Bradley D. E. 1962; A study of the negative staining process.. J. gen. Microbiol 29:503
    [Google Scholar]
  3. Bradley D. E. 1965; The morphology and physiology of bacteriophages as revealed by the electron microscope.. Jl R. Microsc. Soc 84:257
    [Google Scholar]
  4. Bradley D. E. 1966; The structure and infective process of a Pseudomonas aeruginosa bacteriophage containing ribonucleic acid.. J. gen. Microbiol
    [Google Scholar]
  5. Brenner Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses.. Biochem. biophys. Acta 34:103
    [Google Scholar]
  6. Brinton C. C. 1965; The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram negative bacteria.. Trans. N.Y. Acad. Sci 27:1003
    [Google Scholar]
  7. Brinton C. C., Gemski P., Carnahan J. 1964; A new type of bacterial pilus genetically controlled by the fertility factor of E. coli K12 and its role in chromosome transfer.. Proc. natn. Acad. Sci. U.S.A 52:776
    [Google Scholar]
  8. Crawford E. M., Gesteland R. F. 1964; The adsorption of bacteriophage R17.. Virology 22:165
    [Google Scholar]
  9. Edgell M. H., Ginoza W. 1965; The fate during infection of the coat protein of the spherical bacteriophage R17.. Virology 27:23
    [Google Scholar]
  10. Poindexter J. S. 1964; Biological properties and classification of the Caulobacter group.. Bact. Rev 28:231
    [Google Scholar]
  11. Schmidt J. M., Stanier R. Y. 1965; Isolation and characterization of bacteriophages active against stalked bacteria.. J. gen. Microbiol 39:95
    [Google Scholar]
  12. Schmidt J. M., Stanier R. Y. 1966; The development of cellular stalks in bacteria.. J. cell. Biol 28:423
    [Google Scholar]
  13. Stove J. L., Stanier R. Y. 1962; Cellular differentiation in stalked bacteria.. Nature, Lond 196:1189
    [Google Scholar]
  14. Valentine R. C., Wedel H., Ippen K. A. 1965; F-pili requirement for RNA bacteriophage adsorption.. Biochem. biophys. Res. Comm 21:277
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-45-2-347
Loading
/content/journal/micro/10.1099/00221287-45-2-347
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error