SUMMARY: The nutritional requirements for growth on agar media at 28° and at 37° were investigated for strains representative of and Pasteurella strain ‘X’ (). At 28°, strains of either required no growth factors or were dependent on thiamine or pantothenate. Most strains of required cystine, methionine and phenylalanine but some strains could dispense with methionine or phenylalanine while others required additional factors. Strains of Pasteurella ‘X’ either were thiamine-dependent or required no factors. At 37° all strains showed additional requirements. Most strains of could then grow with any 3 of the 4 factors glutamic acid, thiamine, cystine and pantothenate; other strains specifically required all 4 factors and nicotinamide. Strains of grew reliably on media supplemented with cystine, methionine, phenylalanine, glycine, valine, isoleucine, glutamic acid and thiamine when incubated in CO-enriched air. All strains of Pasteurella ‘X’ required thiamine and either cystine or methionine; some substrains showed additional requirements. Virulent and avirulent representative strains of had similar calcium requirements for growth on defined media.


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