1887

Abstract

SUMMARY: A small single-phase continuous fermentor system for convenient longterm operation at steady state with small population densities is described. A method by which the effect of extracellular limiting nutrient on growth rate can be evaluated from the resulting data is discussed. This method was used for to determine the Michaelis constants for growth with oxygen and glycerol; these were found to be 1·4×10 and 4·9×10 . Size of organisms and yields were found to decrease with growth rate. Average volume of organisms decreased from 60 to 41 μ and the yield decreased from 1·8 to 1·2 g. dry wt./organism/g. oxygen consumed. An endogenous metabolism rate constant determined from yields at growth rates above 30% maximum was 0·056 g. oxygen used/g. dry wt. organism/hr.

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/content/journal/micro/10.1099/00221287-45-2-205
1966-11-01
2024-04-25
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References

  1. Adams M. H. 1959 Bacteriophages New York: Interseience Publishers Inc.;
    [Google Scholar]
  2. Amati P., Ozeki H. 1962; Transfer of colicinogenic factors to Serratia marcescens . Abstr. V IIIth int. Congr. Microbiol. Montreal26 Montreal: Thérien Frères Limited.;
    [Google Scholar]
  3. Ben-Gurion R., Hertman I. 1958; Bacteriocin-like material produced by Pasteurella pestis.. J. gen. Microbiol 19:289
    [Google Scholar]
  4. Coetzee J. N. 1968a; Lysogeny in Proteus rettgeri and the host-range of P. rettgeri and P. hauseri bacteriophages.. J. gen. Microbiol 31:219
    [Google Scholar]
  5. Coetzee J. N. 1963b; Transduction of swarming in Proteus mirabilis.. J. gen. Microbiol 33:1
    [Google Scholar]
  6. Fredericq P. 1954; Transduction génétique des propriétés, colicinogènes chez Escherichia coli et Shigella sonnei.. C. r. Séanc. Soc. Biol., Paris 148:399
    [Google Scholar]
  7. Fredericq P. 1955; Induction de la production de colicine et de bacteriophages par irradiation ultraviolette de souches colicinogènes et lysogènes d’Escherichia coli.. C. r. Séanc. Soc. Biol., Paris 149:2028
    [Google Scholar]
  8. Fredericq P. 1958; Colicins and colicinogenic factors.. Symp. Soc. exp. Biol 12:104
    [Google Scholar]
  9. Fuller A. T., Horton J. M. 1950; Marcescin, an antibiotic substance from Serratia marcescens.. J. gen. Microbiol 4:417
    [Google Scholar]
  10. Hamon Y. 1956; Contribution a l’etude des pyocines.. Ann. Inst. Pasteur 91:82
    [Google Scholar]
  11. Hamon Y., Peron Y. 1961; Étude de la propriété bacteriocinogène dans le genre Serratia.. Ann. Inst. Pasteur 100:818
    [Google Scholar]
  12. Hamon Y., Peron Y. 1962; Sur la cinétique de la liberation de leur antibiotique pour les divers types de microbes bacteriocinogènes.. C. r. hebd. Séam. Acad. Sci., Paris 255:2210
    [Google Scholar]
  13. Jacob F., Wollman E. L. 1961 Sexuality and the Genetics of Bacteria New York: Academic Press.;
    [Google Scholar]
  14. Lanni F. 1960; Genetic significance of microbial DNA composition.. Perspect. Biol. Med 3:418
    [Google Scholar]
  15. Levine M. 1957; Mutations in the temperate phage P22 and lysogeny in Salmonella.. Virology 3:22
    [Google Scholar]
  16. Mandel M., Mohn F. A. 1962; Colicin groups of Serratia marcescens . Abstr. VIIIth Int. Congr. Microbiol. Montreal26 Montreal: Thérien Frères Limited.;
    [Google Scholar]
  17. Marmur J., Falkow S., Mandel M. 1963; New approaches to bacterial taxonomy.. A. Rev. Microbiol 17:329
    [Google Scholar]
  18. Martinec T., Kocur M. 1961; The taxonomic status of Serratis marcescens.. Int. Bull. Bact. Nomencl. Taxon 11:7
    [Google Scholar]
  19. Meynell E. 1964; The significance of bacteriophage in bacterial classification.. J. gen. Microbiol 36:461
    [Google Scholar]
  20. Prinsloo H. E., Coetzee J. N. 1964; Host-range of temperate Serratia marcescens bacteriophages.. Nature, Lond 203:211
    [Google Scholar]
  21. Prinsloo H. E., I J., Coetzee J. N. 1965; Agar electrophoresis of antibiotics produced by Serratia marcescens.. Nature, Lond 206:1055
    [Google Scholar]
  22. Ryan F. J., Fried P., Mukoi F. 1965; A colicin produced by cells that are sensitive to it.. Biochim., biophys. Acta 18:131
    [Google Scholar]
  23. Smith S. M., Stocker B. A. D. 1962; Colicinogeny and recombination.. Br. med. Bull 18:46
    [Google Scholar]
  24. Wahba A. H. 1963; The production and inactivation of pyocines.. J. Hyg., Camb 61:431
    [Google Scholar]
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