1887

Abstract

SUMMARY: Some chemical changes which took place in the mycelium of during autolysis were studied. underwent a ‘neutral autolysis’ in which a decrease of 73.9% in mycelial dry weight occurred. Glucose, xylose, maltose and mannitol were found in autolysing mycelium of . The loss of free glucose amounted to 10 mg./flask during the whole period of autolysis. Xylose was decreased to half its initial content by the third day of autolysis and then disappeared by the ninth day, whereas maltose decreased at the beginning and then remained constant from the eighty-first to the hundred and eleventh day of incubation. Eighty-nine per cent of the initial amount of mannitol was lost during the first 9 days of autolysis, whereas fat seemed to be uninvolved in the process. Eighteen different amino acids were found in the autolysing mycelium of . The general picture was of decreasing concentrations during autolysis, and amino acids decreased their content by about 95% with respect to the concentration present at the beginning. This decrease partially accounted for the loss of mycelial-nitrogen observed during autolysis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-45-1-41
1966-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/45/1/mic-45-1-41.html?itemId=/content/journal/micro/10.1099/00221287-45-1-41&mimeType=html&fmt=ahah

References

  1. Arnstein H. R. V., Bentley R. 1953; The biosynthesis of kojic acid. 2. The occurrence of aldolase and triosephosphate isomerase in aspergillus species and their relationship to kojic acid biosynthesis.. Biochem. J 54:508
    [Google Scholar]
  2. Baliga B. R., Krishnamurthy K., Rajagopalan R., Giri K. V. 1955; A simple method for desalting biological fluids for chromatography.. J. Indian Inst. Sci 37:18
    [Google Scholar]
  3. Bent K. J. 1964; Significance of the amino acid pool in nitrogen metabolism of Penicillium griseofulvum.. Biochem. J 92:280
    [Google Scholar]
  4. Bent K. J., Morton A. G. 1964; Effects of external factors on the amino acid pool of Penicillium griseofulvum.. Biochem. J 92:270
    [Google Scholar]
  5. Bentley R. 1957; Preparation and analysis of kojic acid.. Meth. Enzym 3:238
    [Google Scholar]
  6. Chattaway F. W., Toothill C., Barlow A. J. E. 1962; The Amino Acid Metabolism of Microsporum canis.. J. gen. Microbiol 28:721
    [Google Scholar]
  7. Emiliani E., Parera J. M. 1958; Estudio sobre la inductión de la autolysis en una cepa de Aspergillus niger. II. Comunicación: Examen preliminar de los varios grupos de sustancias nitrogenadas e identificatión de los amino acidos del autolizado.. Rev. Fac. Ing. Quim. Univ. Nacl. Litoral, Santa Fe, Arg 27:99
    [Google Scholar]
  8. Emiliani E., deDavie I. 1962; Induced autolysis of Aspergillus phoenicis (A. niger group). IV. Carbohydrates.. Appl. Microbiol 10:504
    [Google Scholar]
  9. Flood A. E., Hirst E. L., Jones J. K. N. 1947; Quantitative estimation of mixtures of sugars by the paper chromatogram method.. Nature, Lond 160:86
    [Google Scholar]
  10. Giri K. V., Radhakrishnan A. N., Vaidyanathan C. S. 1953; Circular paper chromatography. Part VI. The quantitative determination of amino acids.. J. Indian Inst. Sci 35:145
    [Google Scholar]
  11. Jermyn M. A., Isherwood F. A. 1949; Improved separation of sugars on the paper partition chromatogram.. Biochem. J 44:402
    [Google Scholar]
  12. Kay R. E., Harris D. C., Entenman C. 1956; Quantification of the ninhydrin color reaction as applied to paper chromatography.. Arch. Biochem. Biophys 63:14
    [Google Scholar]
  13. Meyers E., Knight S. G. 1961; Studies on the intracellular amino acids of Penicillium roqueforti.. Mycologia 53:115
    [Google Scholar]
  14. Nelson N. 1944; A photometric adaptation of the Somogyi method to the determination of glucose.. J. biol. Chem 153:375
    [Google Scholar]
  15. Partridge S. M. 1948; Filter-paper partition chromatography of sugars. 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep.. Biochem. J 42:238
    [Google Scholar]
  16. Pillai N. C., Srinivasan K. S. 1956; The amino acid metabolism of Aspergillus flavus.. J. gen. Microbiol 14:248
    [Google Scholar]
  17. Ritter R. 1955; Physiologische Untersuchungen an Zygomyceten.. Arch. Mikrobiol 22:248
    [Google Scholar]
  18. Simonart P., Chow K. Y. 1954; Étude du metabolism d’acides amines chez Aspergillus oryzae. III. Acides aminés libres dans le mycelium cultivé sur diverses sources de carbone en presence d’ammonique.. Antonie van Leeuwenhoek 20:174
    [Google Scholar]
  19. Somogyi M. 1945; A new reagent for the determination of sugars.. J. biol. Chem 160:61
    [Google Scholar]
  20. Tandon R. N., Chandra S. 1962; Changes in amino acids, sugars and organic acids in the mycelium of Colletotrichum gloeosporioides Penz. during the autolytic phase of growth.. Phyton 19:127
    [Google Scholar]
  21. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms.. Nature, Lond 166:444
    [Google Scholar]
  22. West D. C., Rapaport S. 1949; Modification of colorimetric method for determination of mannitol and sorbitol in plasma and urine.. Proc. Soc. exp. Biol. Med 70:141
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-45-1-41
Loading
/content/journal/micro/10.1099/00221287-45-1-41
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error