SUMMARY: Some characteristics of protoplast extrusion from hyphae of are described. One, two or more protoplasts might be released from one mycelial compartment. The release was stimulated by controlled dilution of the stabilizing solution. NHCl and mannitol were the best stabilizers of those tested. Connecting threads between protoplasts were observed; their meaning is discussed. Changes in the growth medium did not affect mycelium sensitivity for protoplast formation. Various stages of a protoplast fusion process were followed. Protoplast fusion usually began by an attraction of two bodies followed by junction and fusion to give a single large body. There was no evidence that these fusions represent a sexual process. Fusion of protoplasts in Fusarium is infrequent.


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