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Abstract
SUMMARY: The major characteristics of a temperate phage specific, as far as is known, for one strain of Escherichia coli serotype 04 have been described. It is inactivated by ultraviolet light at a rate characteristic of temperate phages, and it is serologically unrelated to the P1, P22 and T phages. This phage (designated ø 04-CF) is short-tailed, contains DNA, is about 55 mμ, needs no supplemental Ca2+ or Mg2+ for adsorption, shows a latent period of 46 min. and a burst size of 166, and is ultraviolet light inducible. Phage 04-CF can transduce markers to the susceptible indicator strain of E. coli (CF 2004-6). The variety of markers transduced indicates that this is another generalized transducing system for E. coli. The frequencies of transduction lie between 10−6 and 10−8 for different markers, but vary with phage multiplicities. This strain of E. coli also acts as recipient in matings with E. coli K 12 strains.
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