1887

Abstract

SUMMARY: The primary metabolic importance of glutamic acid for phase I was illustrated by its rapid use during the exponential stage of the growth in a chemically defined medium. The pattern of uptake of proline, in particular, and of glutamine resembled that of glutamic acid; this was explained in terms of the conversion of proline and glutamine to glutamic acid. Other substantially used amino acids (alanine, glycine, histidine, serine) were taken up when the readily available sources of glutamic acid became exhausted and the culture entered the stationary phase of growth. The results given show the order of uptake for the amino acids in relation to the growth stage of the culture. No relation was apparent between the amino acid uptake and the immunogenicity of the organisms throughout the growth period.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-44-3-445
1966-09-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/44/3/mic-44-3-445.html?itemId=/content/journal/micro/10.1099/00221287-44-3-445&mimeType=html&fmt=ahah

References

  1. Block R. J. 1950; Estimation of amino acids and amines on paper chromatograms.. Anal. Chem. 22:1327
    [Google Scholar]
  2. Block R. J., Durrum E. L., Zweig G. 1958; Amino acids, amines and proteins.. A Manual of Paper Chromatography and Paper Electrophoresis110 New York: Academic Press Inc.;
    [Google Scholar]
  3. Fukumi H., Sayama E., Tomizawa J., Uchida T. 1953; Nutritional requiremen s and respiratory pattern of pertussis-parapertussis-bronchisepticus group of micro-organisms.. Jap. J. med. Sci. Biol. 6:587
    [Google Scholar]
  4. Goldner M., Jakus C. M., Rhodes H. K., Wilson R. J. 1966; The amino acid utilization by phase I Bordetella pertussis in a chemically defined medium.. J. gen. Microbiol. 44:439
    [Google Scholar]
  5. Jebb W. H. H., Tomlinson A. H. 1951; The catabolic activity of washed suspensions of Haemophilus pertussis.. J. gen. Microbiol. 5:951
    [Google Scholar]
  6. Pekárek J., Stejskal A. 1962; Preparation of non-reactive and non-sensitising vaccine. Round Table Conference on Pertussis Immunisation,. Prague 1:1
    [Google Scholar]
  7. Toennies G., Kolb J. J. 1951; Techniques and reagents for paper chromatography.. Anal. Chem. 23:823
    [Google Scholar]
  8. Wilson R. J. 1963; Cultivation of Bordetella pertussis (phase I) in a chemically-defined medium.. Can. J. pub. Hlth, 54:518
    [Google Scholar]
  9. Worcester J., Wilson E. B. 1943; A table determining LD 50 or the fifty percent end-point.. Proc. natn. Acad. Sci., U.S.A. 29:207
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-44-3-445
Loading
/content/journal/micro/10.1099/00221287-44-3-445
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error