RT Journal Article SR Electronic(1) A1 Goldner, M. A1 Jakus, Cvetka M. A1 Rhodes, Harriett K. A1 Wilson, R. J.YR 1966 T1 The Amino Acid Utilization by Phase I Bordetella pertussis in a Chemically Defined Medium JF Microbiology, VO 44 IS 3 SP 439 OP 444 DO https://doi.org/10.1099/00221287-44-3-439 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Phase I Bordetella pertussis was grown in a chemically defined medium containing 20 amino acids (including glutamine) plus glutathione. Growth was limited by depletion of three of the components, L-glutamic acid, L-proline and L-glutamine. Increasing the concentrations of these three components more than doubled the yield of organism. The following components were utilized, but did not limit growth: alanine, glycine, histidine, serine; cystine and glutathione were also substantially used. Ten amino acids which were not utilized to any significant extent could be omitted without diminishing growth. A preferential order of amino acid utilization is suggested. Glutamic acid appears to be important for antigenicity., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-44-3-439